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PMID: 9689075 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mutation of the polo-box disrupts localization and mitotic functions of the mammalian polo kinase Plk.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 95 ·No. 16 ·1998-08-04 ·Pages 9301-6

Lee KS, Grenfell TZ, Yarm FR, Erikson RL

Abstract

Members of the polo subfamily of protein kinases play pivotal roles in cell proliferation. In addition to the kinase domain, polo kinases have a strikingly conserved sequence in the noncatalytic domain, termed the polo-box. The function of the polo-box is currently undefined. The mammalian polo-like kinase Plk is a functional homologue of Saccharomyces cerevisiae Cdc5. Here, we show that Plk localizes at the spindle poles and cytokinetic neck filaments. Without impairing kinase activity, a conservative mutation in the polo-box disrupts the capacity of Plk to complement the defect associated with a cdc5-1 temperature-sensitive mutation and to localize to these subcellular structures. Our data provide evidence that the polo-box plays a critical role in Plk function, likely by directing its subcellular localization.

MeSH Terms
Amino Acid Sequence Animals Catalysis Cell Cycle Proteins/genetics Fungal Proteins/genetics Genetic Complementation Test Mice Mitosis/genetics Molecular Sequence Data Mutagenesis, Site-Directed Protein Kinases/genetics,metabolism Protein Serine-Threonine Kinases Proto-Oncogene Proteins RNA-Binding Proteins Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Spindle Apparatus
Chemicals
CEF1 protein, S cerevisiae Cell Cycle Proteins Fungal Proteins Proto-Oncogene Proteins RNA-Binding Proteins Saccharomyces cerevisiae Proteins Protein Kinases Protein Serine-Threonine Kinases polo-like kinase 1
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lee K S
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA. kyunglee@pop.nci.nih.gov
Grenfell T Z
Yarm F R
Erikson R L
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31 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.95.16.9301
Email
kyunglee@pop.nci.nih.gov
Published
1998-08-04
Pages
9301-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC21333
Subset
IM
Grants
NCI NIH HHS · CA42580 · United States
External Links
PubMed source DOI: 10.1073/pnas.95.16.9301 PMC Full Text View journal details
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