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PMID: 9689062 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Protein trans-splicing by a split intein encoded in a split DnaE gene of Synechocystis sp. PCC6803.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 95 ·No. 16 ·1998-08-04 ·Pages 9226-31

Wu H, Hu Z, Liu XQ

Abstract

A split intein capable of protein trans-splicing is identified in a DnaE protein of the cyanobacterium Synechocystis sp. strain PCC6803. The N- and C-terminal halves of DnaE (catalytic subunit alpha of DNA polymerase III) are encoded by two separate genes, dnaE-n and dnaE-c, respectively. These two genes are located 745,226 bp apart in the genome and on opposite DNA strands. The dnaE-n product consists of a N-extein sequence followed by a 123-aa intein sequence, whereas the dnaE-c product consists of a 36-aa intein sequence followed by a C-extein sequence. The N- and C-extein sequences together reconstitute a complete DnaE sequence that is interrupted by the intein sequences inside the beta- and tau-binding domains. The two intein sequences together reconstitute a split mini-intein that not only has intein-like sequence features but also exhibited protein trans-splicing activity when tested in Escherichia coli cells.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Cyanobacteria/genetics DNA Polymerase III/genetics DNA Primers Escherichia coli/genetics Genes, Bacterial Molecular Sequence Data Protein Splicing Sequence Homology, Amino Acid
Chemicals
DNA Primers DNA polymerase III, alpha subunit DNA Polymerase III
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wu H
Biochemistry Department, Dalhousie University, Halifax, Nova Scotia, B3H 4H7, Canada.
Hu Z
Liu X Q
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.95.16.9226
Published
1998-08-04
Pages
9226-31
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC21320
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.95.16.9226 PMC Full Text View journal details
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