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PMID: 7988548 Published · ppublish English Journal Article

Protein splicing: an analysis of the branched intermediate and its resolution by succinimide formation.

The EMBO journal ·Vol. 13 ·No. 23 ·1994-12-01 ·Pages 5517-22

Xu MQ, Comb DG, Paulus H, Noren CJ, Shao Y, Perler FB

Abstract

Protein splicing involves the excision of an internal domain from a precursor protein and the ligation of the external domains so as to generate two new proteins. Study of this process has recently been facilitated by the isolation of a precursor and a branched intermediate from a thermophilic protein splicing element expressed in a foreign protein context. Two aspects of protein splicing are examined in this paper. We demonstrate a succinimide at the C-terminus of the spliced internal protein, implicating cyclization of asparagine in resolution of the branched intermediate, and we identify an alkali-labile bond in the branched intermediate. A revised protein splicing model based on these experimental results is presented.

MeSH Terms
Alkalies Amino Acid Sequence Base Sequence Conserved Sequence DNA Primers Molecular Sequence Data Protein Processing, Post-Translational Proteins/chemistry,metabolism Sequence Homology, Amino Acid Succinimides/metabolism
Chemicals
Alkalies DNA Primers Proteins Succinimides succinimide
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Xu M Q
New England Biolabs, Inc., Beverly, MA 01915.
Comb D G
Paulus H
Noren C J
Shao Y
Perler F B
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1994-12-01
Pages
5517-22
Language
English
Region
England
NLM ID
8208664
PMCID
PMC395513
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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