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PMID: 9679143 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Iqg1p, a yeast homologue of the mammalian IQGAPs, mediates cdc42p effects on the actin cytoskeleton.

The Journal of cell biology ·Vol. 142 ·No. 2 ·1998-07-27 ·Pages 443-55

Osman MA, Cerione RA

Abstract

The Rho-type GTPase Cdc42p has been implicated in diverse cellular functions including cell shape, cell motility, and cytokinesis, all of which involve the reorganization of the actin cytoskeleton. Targets of Cdc42p that interface the actin cytoskeleton are likely candidates for mediating cellular activities. In this report, we identify and characterize a yeast homologue for the mammalian IQGAP, a cytoskeletal target for Cdc42p. The yeast IQGAP homologue, designated Iqg1p, displays a two-hybrid interaction with activated Cdc42p and coimmunoprecipitates with actin filaments. Deletion of IQG1 results in a temperature-sensitive lethality and causes aberrant morphologies including elongated and round multinucleated cells. This together with its localization at the mother-bud neck, suggest that Iqg1p promotes budding and cytokinesis. At restrictive temperatures, the vacuoles of the mutant cells enlarge and vesicles accumulate in the bud. Interestingly, Iqg1p shows two-hybrid interactions with the ankyrin repeat-containing protein, Akr1p (Kao, L.-R., J. Peterson, J. Ruiru, L. Bender, and A. Bender. 1996. Mol. Cell. Biol. 16:168-178), which inhibits pheromone signaling and appears to promote cytokinesis and/or trafficking. We also show two-hybrid interactions between Iqg1p and Afr1p, a septin-binding protein involved in projection formation (Konopka, J.B., C. DeMattei, and C. Davis. 1995. Mol. Cell. Biol. 15:723-730). We propose that Iqg1p acts as a scaffold to recruit and localize a protein complex involved in actin-based cellular functions and thus mediates the regulatory effects of Cdc42p on the actin cytoskeleton.

MeSH Terms
Actins/metabolism Amino Acid Sequence Animals Base Sequence Calmodulin/metabolism Cell Cycle Proteins/metabolism Cell Division/genetics Cell Nucleus/metabolism,ultrastructure Cell Polarity/genetics Chitin/metabolism Cytoskeleton/metabolism DNA Primers/genetics Fungal Proteins/genetics,metabolism GTP-Binding Proteins/metabolism GTPase-Activating Proteins Gene Deletion Molecular Sequence Data Proteins/genetics,metabolism Saccharomyces cerevisiae/genetics,growth & development,metabolism Sequence Homology, Amino Acid Temperature cdc42 GTP-Binding Protein, Saccharomyces cerevisiae
Chemicals
Actins Calmodulin Cell Cycle Proteins DNA Primers Fungal Proteins GTPase-Activating Proteins Proteins Chitin GTP-Binding Proteins cdc42 GTP-Binding Protein, Saccharomyces cerevisiae
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Osman M A
Department of Pharmacology, Cornell University, Ithaca, New York 14853, USA.
Cerione R A
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.142.2.443
Published
1998-07-27
Pages
443-55
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2133066
Subset
IM
Grants
NIGMS NIH HHS · GM47458 · United States
External Links
PubMed source DOI: 10.1083/jcb.142.2.443 PMC Full Text View journal details
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