Abstract
The chloramphenicol resistance gene catD from Clostridium difficile was shown to be encoded on the transposons Tn4453a and Tn4453b, which were structurally and functionally related to Tn4451 from Clostridium perfringens. Tn4453a and Tn4453b excised precisely from recombinant plasmids, generating a circular form, as is the case for Tn4451. Evidence that this process is mediated by Tn4453-encoded tnpX genes was obtained from experiments which showed that in trans these genes complemented a Tn4451tnpX delta 1 mutation for excision. Nucleotide sequencing showed that the joint of the circular form generated by the excision of Tn4453a and Tn4453b was similar to that from Tn4451. These results suggest that the Tn4453-encoded TnpX proteins bind to similar DNA target sequences and function in a manner comparable to that of TnpX from Tn4453. Furthermore, it has been shown that Tn4453a and Tn4453b can be transferred to suitable recipient cells by RP4 and therefore are mobilizable transposons. It is concluded that, like Tn4451, they must encode a functional tnpZ gene and a target oriT or RSA site. The finding that related transposable elements are present in C. difficile and C. perfringens has implications for the evolution and dissemination of antibiotic resistance genes and the mobile elements on which they are found within the clostridia.
MeSH Terms
Base Sequence
Chloramphenicol O-Acetyltransferase/genetics
Chloramphenicol Resistance/genetics
Cloning, Molecular
Clostridioides difficile/drug effects,genetics
Clostridium perfringens/genetics
DNA Nucleotidyltransferases/genetics
DNA Transposable Elements/genetics
DNA, Circular/genetics
Escherichia coli
Genetic Complementation Test
Integrases
Molecular Sequence Data
Mutation
Recombinases
Restriction Mapping
Sequence Homology, Nucleic Acid
Chemicals
DNA Transposable Elements
DNA, Circular
Recombinases
Chloramphenicol O-Acetyltransferase
DNA Nucleotidyltransferases
Integrases
integron integrase IntI1
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Lyras D
Department of Microbiology, Monash University, Clayton, Victoria, Australia. dena.lyras@med.monash.edu.au
Storie C
Huggins A S
Crellin P K
Bannam T L
Rood J I
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