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PMID: 9658177 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Multiple functions for actin during filamentous growth of Saccharomyces cerevisiae.

Molecular biology of the cell ·Vol. 9 ·No. 7 ·1998-07-00 ·Pages 1873-89

Cali BM, Doyle TC, Botstein D, Fink GR

Abstract

Saccharomyces cerevisiae is dimorphic and switches from a yeast form to a pseudohyphal (PH) form when starved for nitrogen. PH cells are elongated, bud in a unipolar manner, and invade the agar substrate. We assessed the requirements for actin in mediating the dramatic morphogenetic events that accompany the transition to PH growth. Twelve "alanine scan" alleles of the single yeast actin gene (ACT1) were tested for effects on filamentation, unipolar budding, agar invasion, and cell elongation. Some act1 mutations affect all phenotypes, whereas others affect only one or two aspects of PH growth. Tests of intragenic complementation among specific act1 mutations support the phenotypic evidence for multiple actin functions in filamentous growth. We present evidence that interaction between actin and the actin-binding protein fimbrin is important for PH growth and suggest that association of different actin-binding proteins with actin mediates the multiple functions of actin in filamentous growth. Furthermore, characterization of cytoskeletal structure in wild type and act1/act1 mutants indicates that PH cell morphogenesis requires the maintenance of a highly polarized actin cytoskeleton. Collectively, this work demonstrates that actin plays a central role in fungal dimorphism.

MeSH Terms
Actins/chemistry,genetics,physiology Alleles Cell Division/genetics Cell Polarity/genetics Cytoskeleton/chemistry,genetics,physiology Genes, Dominant/physiology Genetic Complementation Test Membrane Glycoproteins/physiology Microfilament Proteins Models, Molecular Mutagenesis, Site-Directed Nitrogen/deficiency Saccharomyces cerevisiae/cytology,genetics,growth & development
Chemicals
Actins Membrane Glycoproteins Microfilament Proteins plastin Nitrogen
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cali B M
Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA.
Doyle T C
Botstein D
Fink G R
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.9.7.1873
Published
1998-07-00
Pages
1873-89
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC25429
Subset
IM
Grants
NCRR NIH HHS · RR11939-01 · United States
NIGMS NIH HHS · GM35010 · United States
NIGMS NIH HHS · GM46406-07 · United States
External Links
PubMed source DOI: 10.1091/mbc.9.7.1873 PMC Full Text View journal details
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