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PMID: 8603918 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Movement of cortical actin patches in yeast.

The Journal of cell biology ·Vol. 132 ·No. 5 ·1996-03-00 ·Pages 861-70

Waddle JA, Karpova TS, Waterston RH, Cooper JA

Abstract

In yeast, actin forms patches associated with the plasma membrane. Patch distribution correlates with polarized growth during the cell cycle and in response to external stimuli. Using green fluorescent protein fused to capping protein to image actin patches in living cells, we find that patches move rapidly and over long distances. Even patches in clusters, such as at the incipient bud site, show movement. Patches move independently of one another and generally over small distances in a local area, but they can also move larger distances, including through the mother-bud neck. Changes in patch polarization occur quickly through the cell cycle. These observations provide important new parameters for a molecular analysis of the regulation and function of actin.

MeSH Terms
Actins/metabolism Biological Transport Carrier Proteins Cell Compartmentation Cell Cycle Cell Membrane/metabolism Cell Polarity Cytoskeletal Proteins Green Fluorescent Proteins Luminescent Proteins Membrane Proteins Microfilament Proteins Microscopy, Fluorescence Movement Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Time Factors Yeasts/growth & development,metabolism
Chemicals
Actins Cap2 protein, S cerevisiae Carrier Proteins Cytoskeletal Proteins Luminescent Proteins Membrane Proteins Microfilament Proteins Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Green Fluorescent Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Waddle J A
Department of Genetics, Washington University School of Medicine, St. Louis, Missouri, 63110, USA.
Karpova T S
Waterston R H
Cooper J A
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.132.5.861
Published
1996-03-00
Pages
861-70
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120735
Subset
IM
Grants
NIGMS NIH HHS · GM23883 · United States
NIGMS NIH HHS · GM47337 · United States
External Links
PubMed source DOI: 10.1083/jcb.132.5.861 PMC Full Text View journal details
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