Chemical mapping of co-existing RNA structures.
In many cases RNA can assume co-existing or meta-stable structures preventing structure determination by chemical mapping. A novel method is described, by which RNA is modified with dimethyl sulphate without shifting the distribution of different structures. The different structures are then separated in native gel electrophoresis, and structure determination by primer extension can be carried out separately for each structure.
10 references, click to expand
-
Viroid processing: switch from cleavage to ligation is driven by a change from a tetraloop to a loop E conformation.EMBO J. 1997 Feb 3;16(3):599-610 PMID: 9034342
-
Temperature-gradient gel electrophoresis. Thermodynamic analysis of nucleic acids and proteins in purified form and in cellular extracts.Biophys Chem. 1987 May 9;26(2-3):235-46 PMID: 2440495
-
Probing the structure of RNAs in solution.Nucleic Acids Res. 1987 Nov 25;15(22):9109-28 PMID: 2446263
-
Effects of RNA secondary structure on alternative splicing of pre-mRNA: is folding limited to a region behind the transcribing RNA polymerase?Cell. 1988 Jul 29;54(3):393-401 PMID: 2840206
-
Chemical and computer probing of RNA structure.Prog Nucleic Acid Res Mol Biol. 1996;53:131-96 PMID: 8650302
-
Mutations affecting primer RNA interaction with the replication repressor RNA I in plasmid CoIE1: potential RNA folding pathway mutants.EMBO J. 1990 Jan;9(1):295-304 PMID: 1688532
-
Multiple pathways of reversion in viroids for conservation of structural elements.EMBO J. 1993 May;12(5):2129-39 PMID: 8491201
-
The computer simulation of RNA folding pathways using a genetic algorithm.J Mol Biol. 1995 Jun 30;250(1):37-51 PMID: 7541471
-
Only one of four possible secondary structures of the central conserved region of potato spindle tuber viroid is a substrate for processing in a potato nuclear extract.Nucleic Acids Res. 1995 Nov 11;23(21):4246-54 PMID: 7501442
-
RNA synthesis: strategies for the use of bacteriophage RNA polymerases.Gene. 1988 Dec 10;72(1-2):75-89 PMID: 2468576
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: product@genelibs.com
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
