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PMID: 9634584 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Characterization of microtubule binding domains in the Arabidopsis kinesin-like calmodulin binding protein.

The Plant cell ·Vol. 10 ·No. 6 ·1998-06-00 ·Pages 957-65

Narasimhulu SB, Reddy AS

Abstract

The kinesin-like calmodulin binding protein (KCBP) is a new member of the kinesin superfamily that appears to be present only in plants. The KCBP is unique in its ability to interact with calmodulin in a Ca2+-dependent manner. To study the interaction of the KCBP with microtubules, we expressed different regions of the Arabidopsis KCBP and used the purified proteins in cosedimentation assays with microtubules. The motor domain with or without the calmodulin binding domain bound to microtubules. The binding of the motor domain containing the calmodulin binding region to microtubules was inhibited by Ca2+-calmodulin. This Ca2+-calmodulin regulation of motor domain interactions with microtubules was abolished in the presence of antibodies specific to the calmodulin binding region. In addition, the binding of the motor domain lacking the calmodulin binding region to microtubules was not inhibited in the presence of Ca2+-calmodulin, suggesting an essential role for the calmodulin binding region in Ca2+-calmodulin modulation. Results of the cosedimentation assays with the N-terminal tail suggest the presence of a second microtubule binding site on the KCBP. However, the interaction of the N-terminal tail region of the KCBP with microtubules was insensitive to ATP. These data on the interaction of the KCBP with microtubules provide new insights into the functioning of the KCBP in plants.

MeSH Terms
Animals Arabidopsis/metabolism Arabidopsis Proteins Binding Sites Brain/metabolism Calcium/pharmacology Calmodulin/pharmacology Calmodulin-Binding Proteins/chemistry,metabolism Cattle Kinesins/biosynthesis,chemistry,metabolism Microtubules/drug effects,physiology Plant Proteins/chemistry,metabolism Recombinant Proteins/biosynthesis,chemistry,metabolism Tubulin/isolation & purification,metabolism
Chemicals
Arabidopsis Proteins Calmodulin Calmodulin-Binding Proteins KCBP protein, Arabidopsis Plant Proteins Recombinant Proteins Tubulin Kinesins Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Narasimhulu S B
Department of Biology and Program in Cell and Molecular Biology, Colorado State University, Fort Collins, Colorado 80523, USA.
Reddy A S
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.10.6.957
Published
1998-06-00
Pages
957-65
Language
English
Region
England
NLM ID
9208688
PMCID
PMC144043
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.10.6.957 PMC Full Text View journal details
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