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PMID: 9617814 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Molecular cloning and chromosomal mapping of type one serine/threonine protein phosphatases in Arabidopsis thaliana.

Plant molecular biology ·Vol. 37 ·No. 3 ·1998-06-00 ·Pages 471-81

Lin Q, Li J, Smith RD, Walker JC

Abstract

Type one serine/threonine protein phosphatases (PP1s) have been implicated in various processes of plant growth and development. In all plant species studied, PP1s are encoded by multigene families. Previous studies in our laboratory identified five Arabidopsis thaliana PP1 genes (TOPP1, TOPP2, TOPP3, TOPP4 and TOPP5). In the present study, we report the isolation of three additional PP1 genes (TOPP6, TOPP7 and TOPP8). Southern blot analyses indicate that these three newly isolated genes are single-copy genes in A. thaliana genome. All the three genes are expressed in roots, rosettes and flowers, although their expression levels appear to be lower than those of the five previously identified TOPP genes. Six of the eight TOPP genes were mapped to different positions on four of five A. thaliana chromosomes. Sequence comparison revealed that TOPP genes belong to different subgroups of plant PP1 genes, suggesting that they may encode proteins with distinct functions.

MeSH Terms
Amino Acid Sequence Arabidopsis/enzymology,genetics Chromosome Mapping Cloning, Molecular DNA, Complementary Genes, Plant Molecular Sequence Data Phosphoprotein Phosphatases/genetics Phylogeny Recombination, Genetic Sequence Homology, Amino Acid
Chemicals
DNA, Complementary Phosphoprotein Phosphatases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lin Q
Division of Biological Sciences, University of Missouri-Columbia, 65211, USA.
Li J
Smith R D
Walker J C
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1023/a:1005912413555
Published
1998-06-00
Pages
471-81
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
U80920, U80921, U80922
External Links
PubMed source DOI: 10.1023/a:1005912413555 View journal details
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