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PMID: 9617799 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Induction of a beta-phaseolin promoter by exogenous abscisic acid in tobacco: developmental regulation and modulation by external sucrose and Ca2+ ions.

Plant molecular biology ·Vol. 37 ·No. 2 ·1998-05-00 ·Pages 265-74

Bustos MM, Iyer M, Gagliardi SJ

Abstract

Phaseolin genes are induced by unidentified factors at the onset of seed maturation in embryos of both Phaseolus and tobacco. We show that in tobacco, expression of a beta-phaseolin promoter-GUS (PHSbeta-uidA) mRNA and the corresponding GUS activity, could be induced by abscisic acid (ABA). The effect paralleled an increase in the amount of endogenous 12S globulin (Glb12S) mRNA. In contrast, ABA repressed the expression of isocitrate lyase (ll9) mRNA. The responses of PHSbeta-uidA and Glb12S to ABA declined markedly between 11 and 13 DAF, indicating that they are developmentally regulated. We also show evidence that the ABA response of PHSbeta-uidA can be modulated by the external concentrations of sucrose and Ca2+ ion. These compounds inhibited the response if added to the medium separately, in the concentration ranges of 80-200 mM for sucrose and 0.76-20 mM for CaCl2. However, the presence of both sucrose and CaCl2 restored the ABA response to 20-40% of the maximum value measured in sucrose- and CaCl2-free media. These results suggest that ABA induction of beta-phaseolin gene expression is modulated by developmental signals and by the external supply of sucrose and calcium to the embryos.

MeSH Terms
Abscisic Acid/pharmacology Calcium/pharmacology Calcium Chloride/pharmacology Cations, Divalent Gene Expression Regulation, Plant/drug effects,physiology Globulins/genetics Glucuronidase/genetics Isocitrate Lyase/genetics Plant Proteins/genetics Plants, Genetically Modified Plants, Toxic Promoter Regions, Genetic/genetics Recombinant Fusion Proteins Sucrose/pharmacology Tobacco/genetics
Chemicals
Cations, Divalent Globulins Plant Proteins Recombinant Fusion Proteins phaseolin protein, Phaseolus vulgaris Sucrose Abscisic Acid Glucuronidase Isocitrate Lyase Calcium Chloride Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bustos M M
Department of Biological Sciences, University of Maryland Baltimore County, Baltimore 21250, USA.
Iyer M
Gagliardi S J
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1023/a:1005999725715
Published
1998-05-00
Pages
265-74
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source DOI: 10.1023/a:1005999725715 View journal details
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