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PMID: 9614178 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Heat stress activates fission yeast Spc1/StyI MAPK by a MEKK-independent mechanism.

Molecular biology of the cell ·Vol. 9 ·No. 6 ·1998-06-00 ·Pages 1339-49

Shiozaki K, Shiozaki M, Russell P

Abstract

Fission yeast Spc1/StyI MAPK is activated by many environmental insults including high osmolarity, oxidative stress, and heat shock. Spc1/StyI is activated by Wis1, a MAPK kinase (MEK), which is itself activated by Wik1/Wak1/Wis4, a MEK kinase (MEKK). Spc1/StyI is inactivated by the tyrosine phosphatases Pyp1 and Pyp2. Inhibition of Pyp1 was recently reported to play a crucial role in the oxidative stress and heat shock responses. These conclusions were based on three findings: 1) osmotic, oxidative, and heat stresses activate Spc1/StyI in wis4 cells; 2) oxidative stress and heat shock activate Spc1/StyI in cells that express Wis1AA, in which MEKK consensus phosphorylation sites were replaced with alanine; and 3) Spc1/StyI is maximally activated in Deltapyp1 cells. Contrary to these findings, we report: 1) Spc1/StyI activation by osmotic stress is greatly reduced in wis4 cells; 2) wis1-AA and Deltawis1 cells have identical phenotypes; and 3) all forms of stress activate Spc1/StyI in Deltapyp1 cells. We also report that heat shock, but not osmotic or oxidative stress, activate Spc1 in wis1-DD cells, which express Wis1 protein that has the MEKK consensus phosphorylation sites replaced with aspartic acid. Thus osmotic and oxidative stress activate Spc1/StyI by a MEKK-dependent process, whereas heat shock activates Spc1/StyI by a novel mechanism that does not require MEKK activation or Pyp1 inhibition.

MeSH Terms
Binding Sites Calcium-Calmodulin-Dependent Protein Kinases/metabolism Cell Cycle Proteins Conserved Sequence Enzyme Activation Heat-Shock Response/physiology MAP Kinase Kinase Kinases Mitogen-Activated Protein Kinases Osmotic Pressure Oxidative Stress Phosphorylation Protein Serine-Threonine Kinases/genetics,metabolism Protein Tyrosine Phosphatases/genetics,metabolism Schizosaccharomyces/metabolism Schizosaccharomyces pombe Proteins Serine/genetics,metabolism Signal Transduction Threonine/genetics,metabolism
Chemicals
Cell Cycle Proteins Schizosaccharomyces pombe Proteins Threonine Serine Protein Serine-Threonine Kinases Calcium-Calmodulin-Dependent Protein Kinases Mitogen-Activated Protein Kinases sty1 protein, S pombe MAP Kinase Kinase Kinases wis4 protein, S pombe Protein Tyrosine Phosphatases pyp1 protein, S pombe
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Shiozaki K
Departments of Molecular Biology and Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA.
Shiozaki M
Russell P
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.9.6.1339
Published
1998-06-00
Pages
1339-49
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC25354
Subset
IM
Grants
NIGMS NIH HHS · GM-41281 · United States
External Links
PubMed source DOI: 10.1091/mbc.9.6.1339 PMC Full Text View journal details
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