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PMID: 9614175 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The UNI3 gene is required for assembly of basal bodies of Chlamydomonas and encodes delta-tubulin, a new member of the tubulin superfamily.

Molecular biology of the cell ·Vol. 9 ·No. 6 ·1998-06-00 ·Pages 1293-308

Dutcher SK, Trabuco EC

Abstract

We have cloned the UNI3 gene in Chlamydomonas and find that it encodes a new member of the tubulin superfamily. Although Uni3p shares significant sequence identity with alpha-, beta-, and gamma-tubulins, there is a region of Uni3p that has no similarity to tubulins or other known proteins. Mutant uni3-1 cells assemble zero, one, or two flagella. Pedigree analysis suggests that flagellar number in uni3-1 cells is a function of the age of the cell. The uniflagellate uni3-1 cells show a positional phenotype; the basal body opposite the eyespot templates the single flagellum. A percentage of uni3-1 cells also fail to orient the cleavage furrow properly, and basal bodies have been implicated in the placement of cleavage furrows in Chlamydomonas. Finally when uni3-1 cells are observed by electron microscopy, doublet rather than triplet microtubules are observed at the proximal end of the basal bodies. We propose that the Uni3 tubulin is involved in both the function and cell cycle-dependent maturation of basal bodies/centrioles.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Chlamydomonas/genetics,physiology Chromosome Mapping DNA, Complementary Drosophila melanogaster Humans Microtubule Proteins/genetics,metabolism Microtubules/physiology Molecular Sequence Data Plant Proteins Transformation, Genetic Tubulin/genetics,metabolism Xenopus laevis
Chemicals
DNA, Complementary Microtubule Proteins Plant Proteins Tubulin UNI3 protein, Chlamydomonas
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dutcher S K
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Boulder, Colorado 80309-0347, USA.
Trabuco E C
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.9.6.1293
Published
1998-06-00
Pages
1293-308
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC25351
Subset
IM
Grants
NIGMS NIH HHS · GM-32843 · United States
Databases
GENBANK
AF013109
External Links
PubMed source DOI: 10.1091/mbc.9.6.1293 PMC Full Text View journal details
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