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PMID: 9584178 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Processing of the intron-encoded U18 small nucleolar RNA in the yeast Saccharomyces cerevisiae relies on both exo- and endonucleolytic activities.

Molecular and cellular biology ·Vol. 18 ·No. 6 ·1998-06-00 ·Pages 3376-83

Villa T, Ceradini F, Presutti C, Bozzoni I

Abstract

Many small nucleolar RNAs (snoRNAs) are encoded within introns of protein-encoding genes and are released by processing of their host pre-mRNA. We have investigated the mechanism of processing of the yeast U18 snoRNA, which is found in the intron of the gene coding for translational elongation factor EF-1beta. We have focused our analysis on the relationship between splicing of the EF-1beta pre-mRNA and production of the mature snoRNA. Mutations inhibiting splicing of the EF-1beta pre-mRNA have been shown to produce normal U18 snoRNA levels together with the accumulation of intermediates deriving from the pre-mRNA, thus indicating that the precursor is an efficient processing substrate. Inhibition of 5'-->3' exonucleases obtained by insertion of G cassettes or by the use of a rat1-1 xrn1Delta mutant strain does not impair U18 release. In the Exo- strain, 3' cutoff products, diagnostic of an endonuclease-mediated processing pathway, were detected. Our data indicate that biosynthesis of the yeast U18 snoRNA relies on two different pathways, depending on both exonucleolytic and endonucleolytic activities: a major processing pathway based on conversion of the debranched intron and a minor one acting by endonucleolytic cleavage of the pre-mRNA.

MeSH Terms
DNA-Binding Proteins/metabolism Endodeoxyribonucleases/metabolism Exodeoxyribonuclease V Exodeoxyribonucleases/metabolism Exoribonucleases/metabolism Fungal Proteins/genetics,metabolism Introns Peptide Elongation Factor 1 Peptide Elongation Factors/genetics,metabolism RNA Precursors/metabolism RNA Splicing RNA, Small Nuclear/genetics Ribonucleoproteins/genetics,metabolism Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins
Chemicals
DNA-Binding Proteins Fungal Proteins Peptide Elongation Factor 1 Peptide Elongation Factors RNA Precursors RNA, Small Nuclear Ribonucleoproteins Saccharomyces cerevisiae Proteins RAT1 protein, S cerevisiae Endodeoxyribonucleases Exodeoxyribonucleases Exoribonucleases XRN1 protein, S cerevisiae Exodeoxyribonuclease V
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Villa T
Istituto Pasteur-Fondazione Cenci Bolognetti, Dipartimento di Genetica e Biologia Molecolare, Università "La Sapienza," Rome, Italy.
Ceradini F
Presutti C
Bozzoni I
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.18.6.3376
Published
1998-06-00
Pages
3376-83
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC108919
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.18.6.3376 PMC Full Text View journal details
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