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PMID: 9576944 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of a cAMP-dependent protein kinase catalytic subunit required for virulence and morphogenesis in Ustilago maydis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 95 ·No. 10 ·1998-05-12 ·Pages 5684-9

Dürrenberger F, Wong K, Kronstad JW

Abstract

Ustilago maydis, a fungal pathogen of maize, alternates between budding and filamentous growth in response to mating and other environmental signals. Defects in components of the cAMP signaling pathway affect this morphological transition and reveal an association of budding growth with elevated cAMP levels and filamentous growth with low cAMP levels. We have identified two genes, adr1 and uka1, encoding catalytic subunits of cAMP-dependent protein kinase (PKA). Disruption of adr1 resulted in a constitutively filamentous growth phenotype similar to that of mutants deficient in adenylyl cyclase. Importantly, adr1 is required for pathogenicity and is responsible for the majority of PKA activity in fungal cells. In contrast, uka1 has little influence on pathogenicity, and deletion of the uka1 gene does not affect cell morphology. These results provide compelling evidence that regulated PKA activity is crucial during infectious development of U. maydis.

MeSH Terms
Alleles Amino Acid Sequence Catalysis Cyclic AMP/metabolism Cyclic AMP-Dependent Protein Kinases/chemistry,genetics,physiology DNA, Fungal/metabolism Molecular Sequence Data Protein Conformation Sequence Alignment Signal Transduction Structure-Activity Relationship Ustilago/enzymology,growth & development,pathogenicity
Chemicals
DNA, Fungal Cyclic AMP Cyclic AMP-Dependent Protein Kinases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Dürrenberger F
Biotechnology Laboratory, Departments of Microbiology and Immunology, and Plant Science, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
Wong K
Kronstad J W
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.95.10.5684
Published
1998-05-12
Pages
5684-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC20439
Subset
IM
Databases
GENBANK
AF025290
External Links
PubMed source DOI: 10.1073/pnas.95.10.5684 PMC Full Text View journal details
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