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PMID: 8612587 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The pheromone response factor coordinates filamentous growth and pathogenicity in Ustilago maydis.

The EMBO journal ·Vol. 15 ·No. 7 ·1996-04-01 ·Pages 1632-41

Hartmann HA, Kahmann R, Bölker M

Abstract

In Ustilago maydis, the a and b mating type loci regulate cell fusion, filamentous growth and pathogenicity. The a locus encodes a pheromone-based cell recognition system, and the b locus specifies two homeodomain proteins. The expression of all genes in the a and b loci is induced by pheromone. We have identified a HMG protein (Prf1) that binds sequence specifically to pheromone response elements present in the a and b loci. prf1 mutants do not express the a and b genes and are sterile. The disruption of prf1 in pathogenic haploid strains results in a loss of pathogenicity. The constitutive expression of the b genes restores pathogenicity and induces filamentous growth in the absence of the pheromone signal. These results provide evidence that pheromone signalling, filamentous growth and pathogenic development are linked through Prf1.

MeSH Terms
Amino Acid Sequence Base Sequence DNA Primers/genetics DNA, Fungal/genetics Fungal Proteins/genetics,physiology Genes, Fungal Genes, Mating Type, Fungal High Mobility Group Proteins/genetics,physiology Mating Factor Models, Genetic Molecular Sequence Data Mutation Peptides/genetics,physiology Pheromones/genetics,physiology Plant Proteins Signal Transduction Transcription Factors/genetics,physiology Ustilago/genetics,growth & development,pathogenicity
Chemicals
DNA Primers DNA, Fungal Fungal Proteins High Mobility Group Proteins Peptides Pheromones Plant Proteins Transcription Factors pheromone response factor 1, Ustilago maydis Mating Factor
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hartmann H A
Institut für Genetik und Mikrobiologie, Universität München, Germany.
Kahmann R
Bölker M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1996-04-01
Pages
1632-41
Language
English
Region
England
NLM ID
8208664
PMCID
PMC450074
Subset
IM
Databases
GENBANK
U40753
External Links
PubMed source PMC Full Text View journal details
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