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PMID: 9557743 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Recombinant respiratory syncytial virus (RSV) bearing a set of mutations from cold-passaged RSV is attenuated in chimpanzees.

Journal of virology ·Vol. 72 ·No. 5 ·1998-05-00 ·Pages 4467-71

Whitehead SS, Juhasz K, Firestone CY, Collins PL, Murphy BR

Abstract

A set of five missense mutations previously identified by nucleotide sequence analysis of subgroup A cold-passaged (cp) respiratory syncytial virus (RSV) has been introduced into a recombinant wild-type strain of RSV. This recombinant virus, designated rA2cp, appears to replicate less efficiently in the upper and lower respiratory tracts of seronegative chimpanzees than either biologically derived or recombinant wild-type RSV. Infection with rA2cp also resulted in significantly less rhinorrhea and cough than infection with wild-type RSV. These findings confirm the role of the cp mutations in attenuation of RSV and identify their usefulness for inclusion in future live attenuated recombinant RSV vaccine candidates.

MeSH Terms
Animals Base Sequence Cell Line DNA, Viral Humans Molecular Sequence Data Mutation Pan troglodytes Recombination, Genetic Respiratory Syncytial Virus, Human/genetics,pathogenicity
Chemicals
DNA, Viral
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Whitehead S S
Respiratory Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892-0720, USA. sswhitehead@nih.gov
Juhasz K
Firestone C Y
Collins P L
Murphy B R
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22 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.72.5.4467-4471.1998
Email
sswhitehead@nih.gov
Published
1998-05-00
Pages
4467-71
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC109683
Subset
IM
Grants
NIAID NIH HHS · AI-000030 · United States
NIAID NIH HHS · AI-000087 · United States
Databases
GENBANK
AF035006
External Links
PubMed source DOI: 10.1128/JVI.72.5.4467-4471.1998 PMC Full Text View journal details
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