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PMID: 9542950 Published · ppublish English Journal Article

Intravitam diagnosis of human rabies by PCR using saliva and cerebrospinal fluid.

Journal of clinical microbiology ·Vol. 36 ·No. 4 ·1998-04-00 ·Pages 1117-21

Crepin P, Audry L, Rotivel Y, Gacoin A, Caroff C, Bourhy H

Abstract

An optimized reverse transcription (RT)-PCR protocol for the intravitam detection of rabies virus genomic RNA was tested with clinical samples obtained from 28 patients suspected of having rabies, 9 of whom were confirmed to have had rabies by postmortem examination. RT-PCR using saliva combined with an immunofluorescence assay performed with skin biopsy samples allowed detection of rabies in the nine patients.

MeSH Terms
Cerebrospinal Fluid/virology Humans Polymerase Chain Reaction RNA, Viral/analysis Rabies/diagnosis Rabies virus/genetics Saliva/virology
Chemicals
RNA, Viral
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Crepin P
Rabies Unit, Institut Pasteur, Paris, France.
Audry L
Rotivel Y
Gacoin A
Caroff C
Bourhy H
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/JCM.36.4.1117-1121.1998
Published
1998-04-00
Pages
1117-21
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC104702
Subset
IM
External Links
PubMed source DOI: 10.1128/JCM.36.4.1117-1121.1998 PMC Full Text View journal details
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