Comparative field evaluation of the fluorescent-antibody test, virus isolation from tissue culture, and enzyme immunodiagnosis for rapid laboratory diagnosis of rabies.

Journal of clinical microbiology ·Vol. 27 ·No. 3 ·1989-03-00 ·Pages 519-23

Bourhy H, Rollin PE, Vincent J, Sureau P

Abstract

The rabies tissue culture infection test (RTCIT) and rapid rabies enzyme immunodiagnosis (RREID) were compared to the fluorescent-antibody test (FAT) with field specimens. At the French National Reference Center for Rabies, 15,248 specimens were analyzed by FAT and RTCIT, and 2,290 of those specimens were also tested by RREID; 818 other specimens were tested by FAT and RREID in 12 laboratories located in Africa, Asia, and Latin America. The sensitivities and specificities of RREID and RTCIT were comparable. This study showed that both tests can be used as backup procedures to confirm FAT. RREID is also strongly recommended for epidemiological studies and for laboratories which are not equipped for performing FAT.

MeSH Terms

Africa Animals Asia Brain Stem/microbiology Cerebral Cortex/microbiology Culture Techniques Fluorescent Antibody Technique Hippocampus/microbiology Immunoenzyme Techniques Latin America Predictive Value of Tests Rabies/diagnosis,veterinary Rabies virus/immunology,isolation & purification Spectrophotometry

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Bourhy H

National Reference Center for Rabies, World Health Organization Collaborating Centre for Reference and Research on Rabies, Paris, France.

Rollin P E

Vincent J

Sureau P

References (13)

13 references, click to expand

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Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/jcm.27.3.519-523.1989

Published

1989-03-00

Pages

519-23

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC267350

Subset

External Links

PubMed source DOI: 10.1128/jcm.27.3.519-523.1989 PMC Full Text View journal details

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Comparative field evaluation of the fluorescent-antibody test, virus isolation from tissue culture, and enzyme immunodiagnosis for rapid laboratory diagnosis of rabies.

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