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PMID: 9539795 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Functions of the two glutamate transporters GLAST and GLT-1 in the retina.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 95 ·No. 8 ·1998-04-14 ·Pages 4663-6

Harada T, Harada C, Watanabe M, Inoue Y, Sakagawa T, Nakayama N, Sasaki S, Okuyama S, Watase K, Wada K, Tanaka K

Abstract

In the retina, the glutamate transporter GLAST is expressed in Müller cells, whereas the glutamate transporter GLT-1 is found only in cones and various types of bipolar cells. To investigate the functional role of this differential distribution of glutamate transporters, we have analyzed GLAST and GLT-1 mutant mice. In GLAST-deficient mice, the electroretinogram b-wave and oscillatory potentials are reduced and retinal damage after ischemia is exacerbated, whereas GLT-1-deficient mice show almost normal electroretinograms and mild increased retinal damage after ischemia. These results demonstrate that GLAST is required for normal signal transmission between photoreceptors and bipolar cells and that both GLAST and GLT-1 play a neuroprotective role during ischemia in the retina.

MeSH Terms
ATP-Binding Cassette Transporters/analysis,genetics,metabolism Amino Acid Sequence Amino Acid Transport System X-AG Animals Antibodies Biological Transport Electroretinography Ischemia/genetics,pathology,physiopathology Mice Mice, Knockout Molecular Sequence Data Peptide Fragments/chemistry,immunology Retina/cytology,physiology Retinal Vessels
Chemicals
ATP-Binding Cassette Transporters Amino Acid Transport System X-AG Antibodies Peptide Fragments
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Harada T
Department of Degenerative Neurological Diseases, National Institute of Neuroscience, NCNP, Kodaira, Tokyo 187-8502, Japan.
Harada C
Watanabe M
Inoue Y
Sakagawa T
Nakayama N
Sasaki S
Okuyama S
Watase K
Wada K
Tanaka K
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.95.8.4663
Published
1998-04-14
Pages
4663-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC22547
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.95.8.4663 PMC Full Text View journal details
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