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PMID: 7903437 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning and expression of a glutamate transporter from mouse brain.

Neuroscience letters ·Vol. 159 ·No. 1-2 ·1993-09-03 ·Pages 183-6

Tanaka K

Abstract

A complementary DNA clone encoding a transporter for glutamate has been isolated from mouse brain, and its functional properties have been examined in Xenopus oocytes. The transporter displays high affinity for glutamate (Km = 72 microM) and is dependent on external Na+. The cDNA sequence predicts a protein of 543 amino acids with 6 potential transmembrane domains. Northern blot analysis indicates the mRNA encoding the glutamate transporter is expressed both in the brain and in peripheral tissues.

MeSH Terms

Amino Acid Sequence Amino Acid Transport System X-AG Animals Base Sequence Blotting, Northern Brain Chemistry/physiology Cloning, Molecular DNA, Complementary/metabolism DNA-Directed RNA Polymerases/metabolism Glutamates/metabolism Glutamic Acid Glycoproteins/biosynthesis,metabolism Mice Mice, Inbred BALB C Molecular Sequence Data Oocytes/metabolism RNA, Messenger/biosynthesis Rats Sodium/physiology Xenopus

Chemicals

Amino Acid Transport System X-AG DNA, Complementary Glutamates Glycoproteins RNA, Messenger Glutamic Acid Sodium DNA-Directed RNA Polymerases

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Tanaka K

Laboratory for Neural Networks, Institute of Physical and Chemical Research, RIKEN, Saitama, Japan.

Article Info

Journal

Neuroscience letters

Abbr.

Neurosci Lett

ISSN

0304-3940

DOI

10.1016/0304-3940(93)90829-a

Published

1993-09-03

Pages

183-6

Language

English

Region

Ireland

NLM ID

7600130

Subset

External Links

PubMed source DOI: 10.1016/0304-3940(93)90829-a View journal details

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Cloning and expression of a glutamate transporter from mouse brain.

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