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PMID: 9528757 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Dbp6p is an essential putative ATP-dependent RNA helicase required for 60S-ribosomal-subunit assembly in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 18 ·No. 4 ·1998-04-00 ·Pages 1855-65

Kressler D, de la Cruz J, Rojo M, Linder P

Abstract

A previously uncharacterized Saccharomyces cerevisiae open reading frame, YNR038W, was analyzed in the context of the European Functional Analysis Network. YNR038W encodes a putative ATP-dependent RNA helicase of the DEAD-box protein family and was therefore named DBP6 (DEAD-box protein 6). Dbp6p is essential for cell viability. In vivo depletion of Dbp6p results in a deficit in 60S ribosomal subunits and the appearance of half-mer polysomes. Pulse-chase labeling of pre-rRNA and steady-state analysis of pre-rRNA and mature rRNA by Northern hybridization and primer extension show that Dbp6p depletion leads to decreased production of the 27S and 7S precursors, resulting in a depletion of the mature 25S and 5.8S rRNAs. Furthermore, hemagglutinin epitope-tagged Dbp6p is detected exclusively within the nucleolus. We propose that Dbp6p is required for the proper assembly of preribosomal particles during the biogenesis of 60S ribosomal subunits, probably by acting as an rRNA helicase.

MeSH Terms
Amino Acid Sequence Cell Nucleolus/metabolism Cell Survival DEAD-box RNA Helicases Fluorescent Antibody Technique, Indirect Molecular Sequence Data Nucleic Acid Conformation Open Reading Frames Phenotype Proto-Oncogene Proteins RNA Nucleotidyltransferases/chemistry,physiology RNA Precursors/metabolism RNA, Fungal/metabolism RNA, Ribosomal/metabolism RNA, Ribosomal, 5.8S/metabolism Ribosomes/metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins
Chemicals
Proto-Oncogene Proteins RNA Precursors RNA, Fungal RNA, Ribosomal RNA, Ribosomal, 5.8S Saccharomyces cerevisiae Proteins RNA, ribosomal, 25S RNA Nucleotidyltransferases DBP6 protein, S cerevisiae DEAD-box RNA Helicases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kressler D
Département de Biochimie Médicale, Centre Médical Universitaire, Université de Genève, Geneva, Switzerland. dieter.kressler@medecine.unige.ch
de la Cruz J
Rojo M
Linder P
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.18.4.1855
Email
dieter.kressler@medecine.unige.ch
Published
1998-04-00
Pages
1855-65
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC121415
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.18.4.1855 PMC Full Text View journal details
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