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PMID: 9154839 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Rok1p is a putative RNA helicase required for rRNA processing.

Molecular and cellular biology ·Vol. 17 ·No. 6 ·1997-06-00 ·Pages 3398-407

Venema J, Bousquet-Antonelli C, Gelugne JP, Caizergues-Ferrer M, Tollervey D

Abstract

The synthesis of ribosomes involves many small nucleolar ribonucleoprotein particles (snoRNPs) as transacting factors. Yeast strains lacking the snoRNA, snR10, are viable but are impaired in growth and delayed in the early pre-rRNA cleavages at sites A0, A1, and A2, which lead to the synthesis of 18S rRNA. The same cleavages are inhibited by genetic depletion of the essential snoRNP protein Gar1p. Screens for mutations showing synthetic lethality with deletion of the SNR10 gene or with a temperature-sensitive gar1 allele both identified the ROK1 gene, encoding a putative, ATP-dependent RNA helicase of the DEAD-box family. The ROK1 gene is essential for viability, and depletion of Rok1p inhibits pre-rRNA processing at sites A0, A1, and A2, thereby blocking 18S rRNA synthesis. Indirect immunofluorescence by using a ProtA-Rok1p construct shows the protein to be predominantly nucleolar. These results suggest that Rok1p is required for the function of the snoRNP complex carrying out the early pre-rRNA cleavage reactions.

MeSH Terms
Amino Acid Sequence Cell Nucleolus/metabolism Fluorescent Antibody Technique, Indirect Fungal Proteins/genetics Molecular Sequence Data Mutagenesis Nuclear Proteins/genetics RNA Helicases RNA Nucleotidyltransferases/chemistry,genetics,metabolism RNA Precursors/metabolism RNA, Ribosomal, 18S/metabolism RNA, Small Nuclear/metabolism Ribonucleoproteins, Small Nuclear/metabolism Ribonucleoproteins, Small Nucleolar Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Sequence Alignment Zinc Fingers
Chemicals
Fungal Proteins Nuclear Proteins RNA Precursors RNA, Ribosomal, 18S RNA, Small Nuclear Ribonucleoproteins, Small Nuclear Ribonucleoproteins, Small Nucleolar Saccharomyces cerevisiae Proteins GAR1 protein, S cerevisiae RNA Nucleotidyltransferases RNA Helicases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Venema J
European Molecular Biology Laboratory, Heidelberg, Germany. venema@chem.vu.nl
Bousquet-Antonelli C
Gelugne J P
Caizergues-Ferrer M
Tollervey D
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.17.6.3398
Email
venema@chem.vu.nl
Published
1997-06-00
Pages
3398-407
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC232193
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.17.6.3398 PMC Full Text View journal details
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