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PMID: 9512517 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Drosophila myb is required for the G2/M transition and maintenance of diploidy.

Genes & development ·Vol. 12 ·No. 6 ·1998-03-15 ·Pages 831-43

Katzen AL, Jackson J, Harmon BP, Fung SM, Ramsay G, Bishop JM

Abstract

The myb proto-oncogenes are thought to have a role in the cell division cycle. We have examined this possibility by genetic analysis in Drosophila melanogaster, which possesses a single myb gene. We have described previously two temperature-sensitive, recessive lethal mutants in Drosophila myb (Dm myb). The phenotypes of these mutants revealed a requirement for myb in diverse cellular lineages throughout the course of Drosophila development. We now report a cellular explanation for these findings by showing that Dm myb is required for both mitosis and prevention of endoreduplication in wing cells. Myb apparently acts at or near the time of the G2/M transition. The two mutant alleles of Dm myb produce the same cellular phenotype, although the responsible mutations are located in different functional domains of the gene product. The mutant phenotype can be partially suppressed by ectopic expression of either cdc2 or string, two genes that are known to promote the transition from G2 to M. We conclude that Dm myb is required for completion of cell division and may serve two independent functions: promotion of mitosis, on the one hand, and prevention of endoreduplication when cells are arrested in G2, on the other.

MeSH Terms
Amino Acid Sequence Animals Cell Cycle/genetics,physiology Cell Division/genetics,physiology Cell Nucleus/genetics,physiology Cell Survival/genetics,physiology DNA Mutational Analysis DNA Replication/genetics,physiology Diploidy Drosophila melanogaster/genetics G2 Phase/physiology Mitosis/physiology Molecular Sequence Data Mutation/genetics,physiology Phenotype Polyploidy Proto-Oncogene Proteins/genetics,physiology Proto-Oncogene Proteins c-myb Sequence Homology, Amino Acid Temperature Trans-Activators/genetics,physiology Wings, Animal/abnormalities,cytology,physiopathology
Chemicals
Proto-Oncogene Proteins Proto-Oncogene Proteins c-myb Trans-Activators
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Katzen A L
Department of Molecular Genetics, University of Illinois at Chicago, College of Medicine, Chicago, Illinois 60607-7170, USA.
Jackson J
Harmon B P
Fung S M
Ramsay G
Bishop J M
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.12.6.831
Published
1998-03-15
Pages
831-43
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC316624
Subset
IM
Grants
NCI NIH HHS · CA44338 · United States
External Links
PubMed source DOI: 10.1101/gad.12.6.831 PMC Full Text View journal details
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