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PMID: 1438227 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Regulation of BALB/c 3T3 fibroblast proliferation by B-myb is accompanied by selective activation of cdc2 and cyclin D1 expression.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 21 ·1992-11-01 ·Pages 10415-9

Sala A, Calabretta B

Abstract

The B-myb gene is expressed in many cell types at the G1/S transition of the cell cycle. Inhibition of B-myb expression in BALB/c 3T3 fibroblasts by introduction of a B-myb antisense construct greatly diminished cell proliferation, whereas constitutive expression of a human B-myb cDNA in these cells reduced their growth factor requirements and induced a transformed phenotype. Constitutive expression of B-myb cDNA was accompanied by activation of cyclin D1 and cdc2 expression but not of cyclin A and cyclin B. Transfection of BALB/B-myb cells (a cell line expressing high levels of exogenous human B-myb) with a cyclin D1 antisense construct drastically reduced cloning efficiency of these cells. These results suggest that the B-myb-encoded product regulates fibroblast proliferation by activating cdc2 and cyclin D1 gene expression and that abnormal expression of cyclin D1 might be a step in the process of transformation.

Related Genes
A-myb B-myb c-myb cdc2
MeSH Terms
3T3 Cells Animals Base Sequence CDC2 Protein Kinase/biosynthesis,genetics,isolation & purification Cell Division Cloning, Molecular Cyclins/biosynthesis,genetics,isolation & purification DNA, Neoplasm/genetics Gene Expression Humans Kinetics Mice Mice, Inbred BALB C Molecular Sequence Data Oligodeoxyribonucleotides Oligonucleotides, Antisense Oncogene Proteins v-myb Oncogenes Polymerase Chain Reaction/methods Protein-Tyrosine Kinases/genetics RNA, Messenger/isolation & purification,metabolism Retroviridae Proteins, Oncogenic/genetics Transfection Tumor Cells, Cultured
Chemicals
Cyclins DNA, Neoplasm Oligodeoxyribonucleotides Oligonucleotides, Antisense Oncogene Proteins v-myb RNA, Messenger Retroviridae Proteins, Oncogenic Protein-Tyrosine Kinases CDC2 Protein Kinase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sala A
Jefferson Cancer Institute, Thomas Jefferson University, Philadelphia, PA 19107.
Calabretta B
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31 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.21.10415
Published
1992-11-01
Pages
10415-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC50349
Subset
IM
Grants
NCI NIH HHS · CA46782 · United States
NCI NIH HHS · CA56309 · United States
External Links
PubMed source DOI: 10.1073/pnas.89.21.10415 PMC Full Text View journal details
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