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PMID: 9490739 Published · ppublish English Journal Article

The AmMYB308 and AmMYB330 transcription factors from antirrhinum regulate phenylpropanoid and lignin biosynthesis in transgenic tobacco

The Plant cell ·Vol. 10 ·No. 2 ·1998-02-00 ·Pages 135-54

Tamagnone L, Merida A, Parr A, Mackay S, Culianez-Macia FA, Roberts K, Martin C

Abstract

MYB-related transcription factors are known to regulate different branches of flavonoid metabolism in plants and are believed to play wider roles in the regulation of phenylpropanoid metabolism in general. Here, we demonstrate that overexpression of two MYB genes from Antirrhinum represses phenolic acid metabolism and lignin biosynthesis in transgenic tobacco plants. The inhibition of this branch of phenylpropanoid metabolism appears to be specific to AmMYB308 and AmMYB330, suggesting that they recognize their normal target genes in these transgenic plants. Experiments with yeast indicate that AmMYB308 can act as a very weak transcriptional activator so that overexpression may competitively inhibit the activity of stronger activators recognizing the same target motifs. The effects of the transcription factors on inhibition of phenolic acid metabolism resulted in complex modifications of the growth and development of the transgenic plants. The inhibition of monolignol production resulted in plants with at least 17% less lignin in their vascular tissue. This reduction is of importance when designing strategies for the genetic modification of woody crops.

Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Tamagnone
John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom.
Merida
Parr
Mackay
Culianez-Macia
Roberts
Martin
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1532-298X
DOI
10.1105/tpc.10.2.135
Published
1998-02-00
Pages
135-54
Language
English
Region
England
NLM ID
9208688
PMCID
PMC143979
External Links
PubMed source DOI: 10.1105/tpc.10.2.135 PMC Full Text View journal details
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