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PMID: 9489023 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Guard cells possess a calcium-dependent protein kinase that phosphorylates the KAT1 potassium channel.

Plant physiology ·Vol. 116 ·No. 2 ·1998-02-00 ·Pages 785-95

Li J, Lee YR, Assmann SM

Abstract

Increasing evidence suggests that changes in cytosolic Ca2+ levels and phosphorylation play important roles in the regulation of stomatal aperture and as ion transporters of guard cells. However, protein kinases responsible for Ca2+ signaling in guard cells remain to be identified. Using biochemical approaches, we have identified a Ca(2+)-dependent protein kinase with a calmodulin-like domain (CDPK) in guard cell protoplasts of Vicia faba. Both autophosphorylation and catalytic activity of CDPK are Ca2+ dependent. CDPK exhibits a Ca(2+)-induced electrophoretic mobility shift and its Ca(2+)-dependent catalytic activity can be inhibited by the calmodulin antagonists trifluoperazine and N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide. Antibodies to soybean CDPK alpha cross-react with CDPK. Micromolar Ca2+ concentrations stimulate phosphorylation of several proteins from guard cells; cyclosporin A, a specific inhibitor of the Ca(2+)-dependent protein phosphatase calcineurin enhances the Ca(2+)-dependent phosphorylation of several soluble proteins. CDPK from guard cells phosphorylates the K+ channel KAT1 protein in a Ca(2+)-dependent manner. These results suggest that CDPK may be an important component of Ca2+ signaling in guard cells.

MeSH Terms
Calcium/metabolism Catalysis Phosphorylation Plant Cells Plant Proteins/metabolism Plants/enzymology,metabolism Potassium Channels/metabolism Potassium Channels, Inwardly Rectifying Protein Kinases/metabolism
Chemicals
Plant Proteins Potassium Channels Potassium Channels, Inwardly Rectifying Protein Kinases calcium-dependent protein kinase Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Li J
Department of Biology, Pennsylvania State University, University Park 16802, USA.
Lee Y R
Assmann S M
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.116.2.785
Published
1998-02-00
Pages
785-95
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC35138
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.116.2.785 PMC Full Text View journal details
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