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PMID: 9464421 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

An anaerobic, intrachamber incubator for growth of Methanosarcina spp. on methanol-containing solid media.

Applied and environmental microbiology ·Vol. 64 ·No. 2 ·1998-02-00 ·Pages 768-70

Metcalf WW, Zhang JK, Wolfe RS

Abstract

To simplify the incubation of Methanosarcina spp. on solid agar medium, a two-port, manual, rectangular air lock was modified to serve as an anaerobic incubator. In one operation, it is possible to incubate 153 petri plates, the equivalent of 11 standard anaerobic jars, with plating efficiencies identical to those of traditional protocols.

MeSH Terms
Anaerobiosis Methanol/metabolism Methanosarcina/growth & development
Chemicals
Methanol
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Metcalf W W
Department of Microbiology, University of Illinois, Urbana 61801, USA. metcalf@uiuc.edu
Zhang J K
Wolfe R S
References (6)
6 references, click to expand
  1. Methanogens: reevaluation of a unique biological group.
    Microbiol Rev. 1979 Jun;43(2):260-96 PMID: 390357
  2. Genetics in methanogens: transposon insertion mutagenesis of a Methanococcus maripaludis nifH gene.
    J Bacteriol. 1995 Oct;177(20):5773-7 PMID: 7592322
  3. A genetic system for Archaea of the genus Methanosarcina: liposome-mediated transformation and construction of shuttle vectors.
    Proc Natl Acad Sci U S A. 1997 Mar 18;94(6):2626-31 PMID: 9122246
  4. Molecular, genetic, and biochemical characterization of the serC gene of Methanosarcina barkeri Fusaro.
    J Bacteriol. 1996 Oct;178(19):5797-802 PMID: 8824630
  5. Transcriptional regulation in Archaea: in vivo demonstration of a repressor binding site in a methanogen.
    Proc Natl Acad Sci U S A. 1997 Feb 18;94(4):1316-20 PMID: 9037050
  6. Integration of foreign DNA in an intergenic region of the archaeon Methanosarcina mazei without effect on transcription of adjacent genes.
    J Mol Biol. 1996 Sep 13;262(1):12-20 PMID: 8809175
Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/AEM.64.2.768-770.1998
Email
metcalf@uiuc.edu
Published
1998-02-00
Pages
768-70
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC106116
Subset
IM
Grants
NIGMS NIH HHS · F32 GM016504 · United States
NIGMS NIH HHS · 5 F32 GM16504-3 · United States
NIGMS NIH HHS · GM51334 · United States
External Links
PubMed source DOI: 10.1128/AEM.64.2.768-770.1998 PMC Full Text View journal details
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