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PMID: 8824630 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Molecular, genetic, and biochemical characterization of the serC gene of Methanosarcina barkeri Fusaro.

Journal of bacteriology ·Vol. 178 ·No. 19 ·1996-10-00 ·Pages 5797-802

Metcalf WW, Zhang JK, Shi X, Wolfe RS

Abstract

The Methanosarcina barkeri serC gene, encoding phosphoserine aminotransferase, was cloned by complementation of an Escherichia coli serC mutant, and its nucleotide sequence was determined. The M. barkeri SerC protein shares significant homology with other known SerC proteins. E. coli serC hosts carrying the cloned gene express phosphoserine aminotransferase activity, verifying the function of this gene.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Euryarchaeota/enzymology,genetics Genes, Bacterial Genetic Complementation Test Genomic Library Methanosarcina barkeri/enzymology,genetics Molecular Sequence Data Nucleic Acid Hybridization Open Reading Frames RNA, Bacterial/genetics RNA, Messenger/genetics Sequence Analysis, DNA Sequence Homology, Amino Acid Transaminases/genetics
Chemicals
Bacterial Proteins RNA, Bacterial RNA, Messenger Transaminases phosphoserine aminotransferase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Metcalf W W
Department of Microbiology, University of Illinois, Urbana 61801, USA. bill_metcalf@qms1.life.uiuc.edu
Zhang J K
Shi X
Wolfe R S
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.178.19.5797-5802.1996
Email
bill_metcalf@qms1.life.uiuc.edu
Published
1996-10-00
Pages
5797-802
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC178424
Subset
IM
Databases
GENBANK
U51905
External Links
PubMed source DOI: 10.1128/jb.178.19.5797-5802.1996 PMC Full Text View journal details
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