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PMID: 9456315 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation of functional Golgi-derived vesicles with a possible role in retrograde transport.

The Journal of cell biology ·Vol. 140 ·No. 3 ·1998-02-09 ·Pages 541-51

Love HD, Lin CC, Short CS, Ostermann J

Abstract

Secretory proteins enter the Golgi apparatus when transport vesicles fuse with the cis-side and exit in transport vesicles budding from the trans-side. Resident Golgi enzymes that have been transported in the cis-to-trans direction with the secretory flow must be recycled constantly by retrograde transport in the opposite direction. In this study, we describe the functional characterization of Golgi-derived transport vesicles that were isolated from tissue culture cells. We found that under the steady-state conditions of a living cell, a fraction of resident Golgi enzymes was found in vesicles that could be separated from cisternal membranes. These vesicles appeared to be depleted of secretory cargo. They were capable of binding to and fusion with isolated Golgi membranes, and after fusion their enzymatic contents most efficiently processed cargo that had just entered the Golgi apparatus. Those results indicate a possible role for these structures in recycling of Golgi enzymes in the Golgi stack.

MeSH Terms
Adenosine Triphosphate/metabolism Animals Biological Transport CHO Cells Cell Fractionation Coated Vesicles/metabolism Coatomer Protein Cricetinae Cytosol/metabolism Golgi Apparatus/enzymology,metabolism Intracellular Membranes/enzymology,metabolism Membrane Glycoproteins Membrane Proteins/metabolism N-Acetylglucosaminyltransferases/metabolism Organelles/enzymology,metabolism Proteins/metabolism Viral Envelope Proteins/metabolism
Chemicals
Coatomer Protein G protein, vesicular stomatitis virus Membrane Glycoproteins Membrane Proteins Proteins Viral Envelope Proteins Adenosine Triphosphate N-Acetylglucosaminyltransferases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Love H D
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-0146, USA.
Lin C C
Short C S
Ostermann J
References (43)
43 references, click to expand
  1. Reconstitution of the transport of protein between successive compartments of the Golgi measured by the coupled incorporation of N-acetylglucosamine.
    Cell. 1984 Dec;39(2 Pt 1):405-16 PMID: 6498939
  2. Coatomer interaction with di-lysine endoplasmic reticulum retention motifs.
    Science. 1994 Mar 18;263(5153):1629-31 PMID: 8128252
  3. Binding of coatomer to Golgi membranes requires ADP-ribosylation factor.
    J Biol Chem. 1993 Jun 5;268(16):12083-9 PMID: 8505331
  4. SEC21 is a gene required for ER to Golgi protein transport that encodes a subunit of a yeast coatomer.
    Nature. 1992 Dec 10;360(6404):603-5 PMID: 1461285
  5. Bidirectional transport by distinct populations of COPI-coated vesicles.
    Cell. 1997 Jul 25;90(2):335-49 PMID: 9244307
  6. The Golgi complex: in vitro veritas?
    Cell. 1992 Mar 6;68(5):829-40 PMID: 1547485
  7. Distinct sets of SEC genes govern transport vesicle formation and fusion early in the secretory pathway.
    Cell. 1990 May 18;61(4):723-33 PMID: 2188733
  8. Golgi membrane dynamics imaged by freeze-etch electron microscopy: views of different membrane coatings involved in tubulation versus vesiculation.
    Cell. 1993 Oct 8;75(1):123-33 PMID: 8402891
  9. Beta-COP, a 110 kd protein associated with non-clathrin-coated vesicles and the Golgi complex, shows homology to beta-adaptin.
    Cell. 1991 Feb 8;64(3):649-65 PMID: 1840503
  10. Membrane dynamics at the endoplasmic reticulum-Golgi interface.
    J Cell Biol. 1997 Jul 14;138(1):1-4 PMID: 9214376
  11. Glycosyl transferases of baby-hamster-kidney (BHK) cells and ricin-resistant mutants. N-glycan biosynthesis.
    Eur J Biochem. 1981 Jul;117(2):275-84 PMID: 6456144
  12. An integral membrane component of coatomer-coated transport vesicles defines a family of proteins involved in budding.
    Proc Natl Acad Sci U S A. 1995 Aug 15;92(17):8011-5 PMID: 7644530
  13. ER-to-Golgi transport visualized in living cells.
    Nature. 1997 Sep 4;389(6646):81-5 PMID: 9288971
  14. Retention and retrieval in the endoplasmic reticulum and the Golgi apparatus.
    Curr Opin Cell Biol. 1994 Aug;6(4):517-21 PMID: 7986527
  15. Sequential coupling between COPII and COPI vesicle coats in endoplasmic reticulum to Golgi transport.
    J Cell Biol. 1995 Nov;131(4):875-93 PMID: 7490291
  16. Stepwise assembly of functionally active transport vesicles.
    Cell. 1993 Dec 3;75(5):1015-25 PMID: 8252615
  17. Cross-linking of the components of lactose synthetase with dimethylpimelimidate.
    J Biol Chem. 1975 Feb 25;250(4):1434-44 PMID: 234456
  18. Deficient uridine diphosphate-N-acetylglucosamine:glycoprotein N-acetylglucosaminyltransferase activity in a clone of Chinese hamster ovary cells with altered surface glycoproteins.
    J Biol Chem. 1975 May 10;250(9):3303-9 PMID: 1168193
  19. Coatomer is essential for retrieval of dilysine-tagged proteins to the endoplasmic reticulum.
    Cell. 1994 Dec 30;79(7):1199-207 PMID: 8001155
  20. Overlapping distribution of two glycosyltransferases in the Golgi apparatus of HeLa cells.
    J Cell Biol. 1993 Jan;120(1):5-13 PMID: 8416995
  21. Involvement of GTP-binding "G" proteins in transport through the Golgi stack.
    Cell. 1987 Dec 24;51(6):1053-62 PMID: 2826014
  22. Diffusional mobility of Golgi proteins in membranes of living cells.
    Science. 1996 Aug 9;273(5276):797-801 PMID: 8670420
  23. SNARE-mediated retrograde traffic from the Golgi complex to the endoplasmic reticulum.
    Cell. 1996 Apr 19;85(2):205-15 PMID: 8612273
  24. Assembly of asparagine-linked oligosaccharides.
    Annu Rev Biochem. 1985;54:631-64 PMID: 3896128
  25. Coated vesicle assembly in the Golgi requires only coatomer and ARF proteins from the cytosol.
    Nature. 1993 Aug 19;364(6439):732-4 PMID: 8355790
  26. Characteristics of endoplasmic reticulum-derived transport vesicles.
    J Cell Biol. 1994 Sep;126(5):1133-48 PMID: 8063853
  27. Sorting of yeast alpha 1,3 mannosyltransferase is mediated by a lumenal domain interaction, and a transmembrane domain signal that can confer clathrin-dependent Golgi localization to a secreted protein.
    Mol Biol Cell. 1995 Jul;6(7):809-24 PMID: 7579696
  28. Purification of a novel class of coated vesicles mediating biosynthetic protein transport through the Golgi stack.
    Cell. 1989 Jul 28;58(2):329-36 PMID: 2752426
  29. Anterograde transport of algal scales through the Golgi complex is not mediated by vesicles.
    Trends Cell Biol. 1995 Aug;5(8):305-7 PMID: 14732089
  30. Intercompartmental transport in the Golgi complex is a dissociative process: facile transfer of membrane protein between two Golgi populations.
    J Cell Biol. 1984 Jul;99(1 Pt 1):260-71 PMID: 6539782
  31. The reconstitution of intracellular protein transport in cell-free systems.
    Harvey Lect. 1990-1991;86:65-85 PMID: 2152140
  32. A cisternal maturation mechanism can explain the asymmetry of the Golgi stack.
    FEBS Lett. 1997 Sep 8;414(2):177-81 PMID: 9315681
  33. Evidence for recycling of the resident medial/trans Golgi enzyme, N-acetylglucosaminyltransferase I, in ldlD cells.
    J Biol Chem. 1995 Oct 20;270(42):25057-63 PMID: 7559636
  34. Cytosolic ARFs are required for vesicle formation but not for cell-free intra-Golgi transport: evidence for coated vesicle-independent transport.
    Mol Biol Cell. 1994 Feb;5(2):237-52 PMID: 8019009
  35. A coat subunit of Golgi-derived non-clathrin-coated vesicles with homology to the clathrin-coated vesicle coat protein beta-adaptin.
    Nature. 1991 Jan 17;349(6306):215-20 PMID: 1898984
  36. 'Coatomer': a cytosolic protein complex containing subunits of non-clathrin-coated Golgi transport vesicles.
    Nature. 1991 Jan 17;349(6306):248-51 PMID: 1898986
  37. COPII: a membrane coat formed by Sec proteins that drive vesicle budding from the endoplasmic reticulum.
    Cell. 1994 Jun 17;77(6):895-907 PMID: 8004676
  38. Sorting by COP I-coated vesicles under interphase and mitotic conditions.
    J Cell Biol. 1996 Sep;134(6):1411-25 PMID: 8830771
  39. COPI-independent anterograde transport: cargo-selective ER to Golgi protein transport in yeast COPI mutants.
    J Cell Biol. 1997 Feb 24;136(4):789-802 PMID: 9049245
  40. Intracellular aspects of the process of protein synthesis.
    Science. 1975 Aug 1;189(4200):347-58 PMID: 1096303
  41. Localization of a yeast early Golgi mannosyltransferase, Och1p, involves retrograde transport.
    J Cell Biol. 1996 Mar;132(6):985-98 PMID: 8601597
  42. A new type of coated vesicular carrier that appears not to contain clathrin: its possible role in protein transport within the Golgi stack.
    Cell. 1986 Jul 18;46(2):171-84 PMID: 2872969
  43. Evidence that luminal ER proteins are sorted from secreted proteins in a post-ER compartment.
    EMBO J. 1988 Apr;7(4):913-8 PMID: 3402439
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.140.3.541
Published
1998-02-09
Pages
541-51
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2140158
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.140.3.541 PMC Full Text View journal details
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