Postantibiotic effect in Escherichia coli determined with real-time metabolic monitoring.
Metabolic activity was used to quantify the delay in recovery of Escherichia coli after limited exposures to cefotaxime and piperacillin. This was measured with a microphysiometer, a device which measures changes in pH in the cellular environment, which in turn reflects the metabolic activity of the cells. The antibiotics were rapidly pumped into and flushed from each cellular environment. The length of time that the bacteria were exposed to either antibiotic was determined by programmed valve changes. Metabolic activity was measured during periods in which the instrument's pumps were off. Graphical analysis of the data was used to determine the postantibiotic effect. The lengths of the postantibiotic effects of both drugs (95 to 101 min) determined with the microphysiometer corresponded with the reappearance of short, highly motile cells in significant numbers.
15 references, click to expand
-
[Bactericidal effect of piperacillin alone and combined].Presse Med. 1986 Dec 20;15(46):2297-302 PMID: 2949271
-
Functional expression of the Schizosaccharomyces pombe Na+/H+ antiporter gene, sod2, in Saccharomyces cerevisiae.Proc Natl Acad Sci U S A. 1996 May 14;93(10):5031-6 PMID: 8643524
-
Effect on growth curves and killing curves of brief exposure of Escherichia coli to imipenem and piperacillin.J Antimicrob Chemother. 1986 Dec;18 Suppl E:61-5 PMID: 3546248
-
Postantibiotic effect of beta-lactam antibiotics on Escherichia coli evaluated by bioluminescence assay of bacterial ATP.Antimicrob Agents Chemother. 1990 Jan;34(1):102-6 PMID: 2183707
-
Pharmacodynamics of beta-lactam antibiotics on gram-negative bacteria: initial killing, morphology and postantibiotic effect.Scand J Infect Dis Suppl. 1990;74:118-23 PMID: 2129063
-
Postantibiotic effect of CI-960, enoxacin and ciprofloxacin on Escherichia coli: effect on morphology and haemolysin activity.J Antimicrob Chemother. 1992 May;29(5):529-38 PMID: 1320604
-
The cytosensor microphysiometer: biological applications of silicon technology.Science. 1992 Sep 25;257(5078):1906-12 PMID: 1329199
-
The post-antibiotic effect.J Antimicrob Chemother. 1993 Oct;32(4):519-37 PMID: 8288494
-
Novel method for assessing postantibiotic effect by using the Coulter counter.Antimicrob Agents Chemother. 1996 Jul;40(7):1751-3 PMID: 8807079
-
Use of microphysiometry for analysis of heterologous ion channels expressed in yeast.Nat Biotechnol. 1996 Jul;14(7):880-3 PMID: 9631015
-
Microfabrication in silicon microphysiometry.Clin Chem. 1994 Sep;40(9):1800-4 PMID: 8070104
-
Postantibiotic effect of meropenem on members of the family Enterobacteriaceae determined by five methods.Antimicrob Agents Chemother. 1994 Nov;38(11):2583-9 PMID: 7872752
-
Decreased bactericidal activity during the period of the postantibiotic effect.J Antimicrob Chemother. 1994 Dec;34(6):921-30 PMID: 7730235
-
Control-related effective regrowth time and post-antibiotic effect of meropenem on gram-negative bacteria studied by bioluminescence and viable counts.J Antimicrob Chemother. 1995 May;35(5):585-92 PMID: 7592171
-
Postantibiotic effect of imipenem on gram-positive and gram-negative micro-organisms.J Antimicrob Chemother. 1986 Dec;18 Suppl E:47-59 PMID: 3546247
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