Home LiteratureArticle Details
PMID: 9422608 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Diacylglycerol kinase is involved in regulation of expression of the lantibiotic mutacin II of Streptococcus mutans.

Journal of bacteriology ·Vol. 180 ·No. 1 ·1998-01-00 ·Pages 167-70

Chen P, Novak J, Qi F, Caufield PW

Abstract

Genetic characterization of a Tn916 transposon mutant, Streptococcus mutans T8-1, defective in mutacin II production, revealed that the transposon was inserted into the 3' region of a diacylglycerol kinase (dgk) gene. The insertion occurred in the same region as described for another S. mutans mutant, GS5Tn1, which was altered in its ability to respond to environmental stress (Y. Yamashita, T. Takehara, and H. K. Kuramitsu, J. Bacteriol. 175:6220-6228, 1993). Quantitative primer extension from the mutacin structural gene mutA showed a decreased level (about eightfold) of mutA transcription for mutant T8-1. Mutacin production was restored by transforming mutant T8-1 with integration vector pVA891 containing an intact dgk gene. These data indicated that the full-length dgk gene product along with the mutacin biosynthetic operon are required for the production of the mutacin II lantibiotic.

MeSH Terms
Amino Acid Sequence Bacteriocins/biosynthesis Base Sequence Cloning, Molecular Diacylglycerol Kinase/genetics,physiology Gene Dosage Gene Expression Regulation, Bacterial/physiology Genes, Bacterial/genetics Molecular Sequence Data Mutagenesis, Insertional Protein Precursors/genetics RNA, Bacterial/analysis RNA, Messenger/analysis Streptococcus mutans/enzymology,genetics Transcription, Genetic/genetics
Chemicals
Bacteriocins Protein Precursors RNA, Bacterial RNA, Messenger mutacin II Diacylglycerol Kinase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Chen P
Department of Oral Biology, School of Dentistry, University of Alabama at Birmingham, 35294, USA.
Novak J
Qi F
Caufield P W
References (34)
34 references, click to expand
  1. Insertional mutagenesis and recovery of interrupted genes of Streptococcus mutans by using transposon Tn917: preliminary characterization of mutants displaying acid sensitivity and nutritional requirements.
    J Bacteriol. 1996 Jul;178(14):4166-75 PMID: 8763945
  2. sn-1,2-diacylglycerol kinase of Escherichia coli. Diacylglycerol analogues define specificity and mechanism.
    J Biol Chem. 1990 Mar 15;265(8):4374-81 PMID: 2155227
  3. Bacteriocins from human and rodent streptococci.
    Arch Oral Biol. 1969 Mar;14(3):251-8 PMID: 4978137
  4. Production and properties of bacteriocins (mutacins) from Streptococcus mutans.
    Arch Oral Biol. 1975 Oct;20(10):641-8 PMID: 1059386
  5. Bacteriocins as factors in the in vitro interaction between oral streptococci in plaque.
    Infect Immun. 1977 Jun;16(3):773-80 PMID: 892898
  6. Evidence for a chromosome-borne resistance transposon (Tn916) in Streptococcus faecalis that is capable of "conjugal" transfer in the absence of a conjugative plasmid.
    J Bacteriol. 1981 Jan;145(1):494-502 PMID: 6257641
  7. Role of bacteriocin during plaque formation by Streptococcus salivarius and Streptococcus sanguis on a tooth in an artificial mouth.
    J Appl Bacteriol. 1981 Apr;50(2):305-13 PMID: 7251515
  8. LexA protein is a repressor of the colicin E1 gene.
    J Biol Chem. 1983 Nov 10;258(21):13258-61 PMID: 6355108
  9. Novel shuttle plasmid vehicles for Escherichia-Streptococcus transgeneric cloning.
    Gene. 1983 Nov;25(1):145-50 PMID: 6319229
  10. Isolation of a Streptococcus mutans strain producing a novel bacteriocin.
    Infect Immun. 1984 Apr;44(1):141-4 PMID: 6706403
  11. Distinct bacteriocin groups correlate with different groups of Streptococcus mutans plasmids.
    Infect Immun. 1985 Apr;48(1):51-6 PMID: 2984124
  12. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  13. Role of Streptococcus mutans in human dental decay.
    Microbiol Rev. 1986 Dec;50(4):353-80 PMID: 3540569
  14. Identification of a gene that regulates expression of M protein, the major virulence determinant of group A streptococci.
    Proc Natl Acad Sci U S A. 1987 Dec;84(23):8677-81 PMID: 2446327
  15. Prepeptide sequence of epidermin, a ribosomally synthesized antibiotic with four sulphide-rings.
    Nature. 1988 May 19;333(6170):276-8 PMID: 2835685
  16. Genome walking by single-specific-primer polymerase chain reaction: SSP-PCR.
    Gene. 1989 Dec 7;84(1):1-8 PMID: 2691331
  17. Diacylglycerol kinase: a key modulator of signal transduction?
    Trends Biochem Sci. 1990 Feb;15(2):47-50 PMID: 2159661
  18. Preliminary characterization of four bacteriocins from Streptococcus mutans.
    Can J Microbiol. 1990 Feb;36(2):123-30 PMID: 2337834
  19. Transposition of Tn916 to different sites in the chromosome of Neisseria meningitidis: a genetic tool for meningococcal mutagenesis.
    Mol Microbiol. 1990 May;4(5):729-35 PMID: 2167422
  20. Use of transposon Tn916 to inactivate and isolate a mutacin-associated gene from Streptococcus mutans.
    Infect Immun. 1990 Dec;58(12):4126-35 PMID: 2174834
  21. Genome walking by single specific primer-polymerase chain reaction.
    Methods Enzymol. 1993;217:436-46 PMID: 8474344
  22. Molecular characterization of a STreptococcus mutans mutant altered in environmental stress responses.
    J Bacteriol. 1993 Oct;175(19):6220-8 PMID: 8407794
  23. Enhanced transformation of Streptococcus mutans by modifications in culture conditions.
    Anal Biochem. 1993 Oct;214(1):343-6 PMID: 8250246
  24. Effects of transcriptional start site sequence and position on nucleotide-sensitive selection of alternative start sites at the pyrC promoter in Escherichia coli.
    J Bacteriol. 1994 May;176(10):2938-45 PMID: 7910603
  25. Isolation and biochemical characterization of a novel lantibiotic mutacin from Streptococcus mutans.
    J Bacteriol. 1994 Jul;176(14):4316-20 PMID: 8021218
  26. Membrane topology of Escherichia coli diacylglycerol kinase.
    J Bacteriol. 1994 Sep;176(17):5459-65 PMID: 8071224
  27. Biosynthesis and biological activities of lantibiotics with unique post-translational modifications.
    Eur J Biochem. 1995 Jun 15;230(3):827-53 PMID: 7601145
  28. Mutacin II, a bactericidal antibiotic from Streptococcus mutans.
    Antimicrob Agents Chemother. 1995 Dec;39(12):2656-60 PMID: 8592997
  29. Exploring the allowed sequence space of a membrane protein.
    Nat Struct Biol. 1996 Feb;3(2):141-8 PMID: 8564540
  30. Extraction of RNA from gram-positive bacteria.
    Biotechniques. 1995 Dec;19(6):880-84 PMID: 8747648
  31. Detection of modified amino acids in lantibiotic peptide mutacin II by chemical derivation and electrospray ionization-mass spectroscopic analysis.
    Anal Biochem. 1996 May 1;236(2):358-60 PMID: 8660519
  32. Expression of Serratia marcescens extracellular proteins requires recA.
    J Bacteriol. 1990 Jan;172(1):342-9 PMID: 2152908
  33. Sequence analysis of termini of conjugative transposon Tn916.
    J Bacteriol. 1988 Jul;170(7):3046-52 PMID: 2838457
  34. Sequence analysis of mutA and mutM genes involved in the biosynthesis of the lantibiotic mutacin II in Streptococcus mutans.
    Gene. 1998 Jan 5;206(1):37-43 PMID: 9461412
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.180.1.167-170.1998
Published
1998-01-00
Pages
167-70
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC106864
Subset
IM
Grants
NIAID NIH HHS · P30 AI027767 · United States
NIAID NIH HHS · P30 AI27767 · United States
NIDCR NIH HHS · R01 DE009082 · United States
NCI NIH HHS · P30 CA013148 · United States
NIDCR NIH HHS · DE09082 · United States
NCI NIH HHS · CA13148 · United States
Databases
GENBANK
AF000954
External Links
PubMed source DOI: 10.1128/JB.180.1.167-170.1998 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2