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PMID: 9421526 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Human RPA (hSSB) interacts with EBNA1, the latent origin binding protein of Epstein-Barr virus.

Nucleic acids research ·Vol. 26 ·No. 2 ·1998-01-15 ·Pages 631-7

Zhang D, Frappier L, Gibbs E, Hurwitz J, O'Donnell M

Abstract

RPA is the replicative single-strand DNA (ssDNA) binding protein of eukaryotic chromosomes. This report shows that human RPA interacts with EBNA1, the latent origin binding protein of Epstein-Barr virus (EBV). RPA binds to EBNA1 both in solution, and when EBNA1 is bound to the EBV origin. RPA is a heterotrimer, and the main contact with EBNA1 is formed through the 70 kDa subunit of RPA, the subunit which binds to ssDNA. We propose that this interaction between RPA and EBNA1 is an early step in activation of the latent origin of EBV.

MeSH Terms
DNA, Viral/chemistry,metabolism DNA-Binding Proteins/genetics,metabolism Dimerization Epstein-Barr Virus Nuclear Antigens/genetics,metabolism Escherichia coli/genetics Herpesvirus 4, Human/genetics Humans Peptide Fragments/genetics,metabolism Recombinant Proteins/metabolism Replication Protein A
Chemicals
DNA, Viral DNA-Binding Proteins Epstein-Barr Virus Nuclear Antigens Peptide Fragments RPA1 protein, human Recombinant Proteins Replication Protein A
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Zhang D
Laboratory of DNA replication, The Rockefeller University, Howard Hughes Medical Institute, 1230 York Avenue, New York, NY 10021, USA.
Frappier L
Gibbs E
Hurwitz J
O'Donnell M
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/26.2.631
Published
1998-01-15
Pages
631-7
Language
English
Region
England
NLM ID
0411011
PMCID
PMC147273
Subset
IM
Grants
NIGMS NIH HHS · GM38559 · United States
NIGMS NIH HHS · GM54705 · United States
External Links
PubMed source DOI: 10.1093/nar/26.2.631 PMC Full Text View journal details
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