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PMID: 9396743 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A chromatin-associated kinesin-related protein required for normal mitotic chromosome segregation in Drosophila.

The Journal of cell biology ·Vol. 139 ·No. 6 ·1997-12-15 ·Pages 1361-71

Molina I, Baars S, Brill JA, Hales KG, Fuller MT, Ripoll P

Abstract

The tiovivo (tio) gene of Drosophila encodes a kinesin-related protein, KLP38B, that colocalizes with condensed chromatin during cell division. Wild-type function of the tio gene product KLP38B is required for normal chromosome segregation during mitosis. Mitotic cells in tio larval brains displayed circular mitotic figures, increased ploidy, and abnormal anaphase figures. KLP38B mRNA is maternally provided and expressed in cells about to undergo division. We propose that KLP38B, perhaps redundantly with other chromosome-associated microtubule motor proteins, contributes to interactions between chromosome arms and microtubules important for establishing bipolar attachment of chromosomes and assembly of stable bipolar spindles.

MeSH Terms
Amino Acid Sequence Anaphase Animals Brain/cytology,metabolism Cell Division Chromatin/physiology,ultrastructure Chromosomes/physiology Drosophila/genetics Drosophila Proteins Genes, Insect Insect Proteins/biosynthesis,genetics Introns Kinesins/biosynthesis,chemistry,genetics Larva Mitosis Molecular Sequence Data Ploidies RNA, Messenger/biosynthesis Restriction Mapping Sequence Alignment Sequence Homology, Amino Acid Transcription, Genetic
Chemicals
Chromatin Drosophila Proteins Insect Proteins RNA, Messenger neb protein, Drosophila Kinesins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Molina I
Centro de Biología Molecular "Severo Ochoa" Consejo Superior Investigaciones Científicas-Universidad Autónoma Madrid, Facultad de Ciencias, UAM, Cantoblanco, 28049 Madrid, Spain.
Baars S
Brill J A
Hales K G
Fuller M T
Ripoll P
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.139.6.1361
Published
1997-12-15
Pages
1361-71
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2132616
Subset
IM
Grants
NICHD NIH HHS · 5-F32-HD07728 · United States
NICHD NIH HHS · 5R01-HD29194 · United States
Databases
GENBANK
Y15427
External Links
PubMed source DOI: 10.1083/jcb.139.6.1361 PMC Full Text View journal details
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