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PMID: 9230081 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

KLP38B: a mitotic kinesin-related protein that binds PP1.

The Journal of cell biology ·Vol. 138 ·No. 2 ·1997-07-28 ·Pages 395-409

Alphey L, Parker L, Hawcroft G, Guo Y, Kaiser K, Morgan G

Abstract

We have identified a new member of the kinesin superfamily in Drosophila, KLP38B (kinesin-like protein at 38B). KLP38B was isolated through its two-hybrid interaction with the catalytic subunit of type 1 serine/threonine phosphoprotein phosphatase (PP1). We demonstrate that recombinant KLP38B and PP1 associate in vitro. This is the first demonstration of direct binding of a kinesin-related protein to a regulatory enzyme. Though most closely related to the Unc-104 subfamily of kinesin-related proteins, KLP38B is expressed only in proliferating cells. KLP38B mutants show cell proliferation defects in many tissues. KLP38B is required for normal chromatin condensation as embryos from KLP38B mutant mothers have undercondensed chromatin at metaphase and anaphase. This is the first time that a kinesin-related protein has been shown to have such a role. Incomplete lethality of a strong KLP38B allele suggests partial redundancy with one or more additional kinesin-related proteins.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Cycle Cell Division Cloning, Molecular DNA, Complementary/genetics Drosophila/enzymology,genetics Drosophila Proteins Female Fertility Gene Expression Regulation, Enzymologic Kinesins/genetics,metabolism,physiology Male Mitosis/physiology Molecular Sequence Data Mutation Organ Specificity Phosphoprotein Phosphatases/metabolism RNA, Messenger/analysis Restriction Mapping Sequence Analysis, DNA Sequence Homology, Amino Acid
Chemicals
DNA, Complementary Drosophila Proteins RNA, Messenger neb protein, Drosophila Phosphoprotein Phosphatases Kinesins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Alphey L
School of Biological Sciences, University of Manchester, Manchester M13 9PT, UK. Luke.Alphey@man.ac.uk
Parker L
Hawcroft G
Guo Y
Kaiser K
Morgan G
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.138.2.395
Email
luke.alphey@man.ac.uk
Published
1997-07-28
Pages
395-409
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2138191
Subset
IM
Databases
GENBANK
X99617
External Links
PubMed source DOI: 10.1083/jcb.138.2.395 PMC Full Text View journal details
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