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PMID: 7730406 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The Drosophila kinesin-like protein KLP3A is a midbody component required for central spindle assembly and initiation of cytokinesis.

The Journal of cell biology ·Vol. 129 ·No. 3 ·1995-05-00 ·Pages 709-23

Williams BC, Riedy MF, Williams EV, Gatti M, Goldberg ML

Abstract

We describe here a new member of the kinesin superfamily in Drosophila, KLP3A (Kinesin-Like-Protein-at-3A). The KLP3A protein localizes to the equator of the central spindle during late anaphase and telophase of male meiosis. Mutations in the KLP3A gene disrupt the interdigitation of microtubules in spermatocyte central spindles. Despite this defect, anaphase B spindle elongation is not obviously aberrant. However, cytokinesis frequently fails after both meiotic divisions in mutant testes. Together, these findings strongly suggest that the KLP3A presumptive motor protein is a critical component in the establishment or stabilization of the central spindle. Furthermore, these results imply that the central spindle is the source of signals that initiate the cleavage furrow in higher cells.

Related Genes
KLP3A
MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Compartmentation Cell Division/physiology Cloning, Molecular Drosophila/cytology,physiology,ultrastructure Drosophila Proteins Female Fertility Genes, Insect/genetics Immunoblotting Immunohistochemistry Kinesins/genetics,immunology,physiology Male Meiosis/physiology Microscopy, Video Microtubules/physiology,ultrastructure Molecular Sequence Data Mutation Recombinant Proteins/metabolism Sequence Analysis, DNA Sex Characteristics Spermatocytes/physiology,ultrastructure Spindle Apparatus/physiology,ultrastructure
Chemicals
Drosophila Proteins Klp3A protein, Drosophila Recombinant Proteins Kinesins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Williams B C
Section of Genetics and Development, Cornell University, Ithaca, New York 14853-2703, USA.
Riedy M F
Williams E V
Gatti M
Goldberg M L
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.129.3.709
Published
1995-05-00
Pages
709-23
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120429
Subset
IM
Grants
NIGMS NIH HHS · GM48430 · United States
Databases
GENBANK
L19117
External Links
PubMed source DOI: 10.1083/jcb.129.3.709 PMC Full Text View journal details
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