Home LiteratureArticle Details
PMID: 938478 Published · ppublish English Journal Article

Human lysosomal elastase. Catalytic and immunological properties.

The Biochemical journal ·Vol. 155 ·No. 2 ·1976-05-01 ·Pages 265-71

Starkey PM, Barrett AJ

Abstract

1. The elastase of human spleen was shown to exhibit endopeptidase activity against azo-casein and elastin. 2. Activity against several synthetic substrates was detected, and benzyloxycarbonyl-L-alanine 2-naphthyl ester was found to be a good substrate for routine use. 3. The enzyme showed a broad pH optimum in the range of 8.2-9.2 against azo-casein and the synthetic substrate. 4. The effect of inhibitors on the spleen elastase showed it to be a serine proteinase with a specificity similar to that of porcine pancreatic elastase. 5. Specific antisera were raised against the enzyme, and it was shown to be immunologically identical with the lysosomal elastase of human neutrophil leucocytes.

MeSH Terms
Caseins Elastin Humans Immune Sera Lysosomes/enzymology Neutrophils/enzymology Pancreatic Elastase/antagonists & inhibitors,immunology,metabolism Spleen/enzymology
Chemicals
Caseins Immune Sera Elastin Pancreatic Elastase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Starkey P M
Barrett A J
References (40)
40 references, click to expand
  1. The preparation and properties of two new chromogenic substrates of trypsin.
    Arch Biochem Biophys. 1961 Nov;95:271-8 PMID: 13890599
  2. Chloroacyl esters as histochemical substrates.
    J Histochem Cytochem. 1953 Nov;1(6):469-70 PMID: 13118129
  3. The effect of gold salt on lysosomal enzymes of the peritoneal macrophage.
    Arthritis Rheum. 1966 Feb;9(1):57-65 PMID: 5006477
  4. Histochemical characterization of cellular and structural elements of the human spleen.
    J Histochem Cytochem. 1972 Dec;20(12):1049-58 PMID: 4118358
  5. Active-site specific inhibitors of elastase.
    Biochemistry. 1973 Nov 6;12(23):4767-74 PMID: 4797992
  6. Electrophoresis of elastase-like esterases from human neutrophils.
    J Histochem Cytochem. 1974 May;22(5):327-39 PMID: 4133796
  7. The synthesis and analytical use of a highly sensitive and convenient substrate of elastase.
    Biochem Med. 1974 Dec;11(4):350-7 PMID: 4429553
  8. Chicken alpha2-proteinase inhibitor: a serum protein homologous with ovoinhibitor of egg white.
    Biochim Biophys Acta. 1974 Nov 5;371(1):52-62 PMID: 4214561
  9. Isolation of elastase-like and chymotrypsin-like neutral proteases from human granulocytes.
    Hoppe Seylers Z Physiol Chem. 1974 Sep;355(9):1077-82 PMID: 4461651
  10. The neutral proteases of human granulocytes. Isolation and partial characterization of granulocyte elastases.
    Eur J Biochem. 1974 Mar 1;42(2):519-27 PMID: 4208353
  11. A new radioactive assay for enzymes with elastolytic activity using reduced tritiated elastin. The effect of sodium dodecyl sulfate on elastolysis.
    Biochim Biophys Acta. 1973 Nov 15;327(1):138-45 PMID: 4770737
  12. The effect of human granulocyte elastase on bacterial suspensions.
    Lab Invest. 1973 Oct;29(4):454-7 PMID: 4200659
  13. Regulation of elastase-catalyzed hydrolysis of insoluble elastin by synthetic and naturally occurring hydrophobic ligands.
    Biochemistry. 1974 Aug 13;13(17):3497-503 PMID: 4846292
  14. Histochemical azo coupling reactions of the pigments of obstructive icterus and of hematoidin. I. Diazonium salts used.
    J Histochem Cytochem. 1969 Nov;17(11):738-48 PMID: 4195387
  15. Inhibition of porcine elastase by turkey ovomucoid and chicken ovoinhibitor.
    Eur J Biochem. 1971 Jun 29;20(4):547-52 PMID: 5104189
  16. The non-specific electrostatic nature of the adsorption of elastase and other basic proteins on elastin.
    Eur J Biochem. 1971 Jun 29;20(4):541-6 PMID: 5104188
  17. Inhibition of human granulocyte elastase by gold sodium thiomalate.
    Biochem Pharmacol. 1970 Feb;19(2):626-8 PMID: 5507673
  18. Elastase-like, esteroprotease activity in human and rabbit alveolar macrophage granules.
    Proc Soc Exp Biol Med. 1971 Apr;136(4):1054-8 PMID: 5554445
  19. Further studies on elastase-like esterases in human leukocyte granules.
    Proc Soc Exp Biol Med. 1971 Apr;136(4):1045-9 PMID: 5554444
  20. The use of p-nitrophenyl N-tert-butyloxycarbonyl-L-alaninate as substrate for elastase.
    Biochim Biophys Acta. 1972 Apr 7;268(1):257-60 PMID: 5062949
  21. Acetyl-L-alanyl-L-alanyl-L-alanine methyl ester: a new highly specific elastase substrate.
    Can J Biochem. 1970 Mar;48(3):384-6 PMID: 5461953
  22. Alanine p-nitrophenyl esterase activity of human leucocyte granules.
    Biochem J. 1969 Aug;114(1):157-9 PMID: 5810053
  23. Human granulocyte elastase. Further delineation of its role in connective tissue damage.
    Am J Pathol. 1972 Sep;68(3):579-92 PMID: 4342329
  24. Studies on a human blood platelet protease with elastolytic activity.
    Biochim Biophys Acta. 1973 Jun 6;309(2):406-13 PMID: 4731969
  25. Neutrophil proteases in inflammation.
    Annu Rev Med. 1972;23:177-90 PMID: 4345523
  26. The action of elastase on p-nitroanilide substrates.
    Biochem Biophys Res Commun. 1973 Jul 17;53(2):383-90 PMID: 4736813
  27. N-acetyl-(L-Ala) 3 -p-nitroanilide as a new chromogenic substrate for elastase.
    Biochem Biophys Res Commun. 1973 Feb 20;50(4):1020-6 PMID: 4734817
  28. Neutral protease from the polymorphonuclear leucocytes of human rheumatoid synovial fluid.
    Clin Sci Mol Med. 1974 Nov;47(5):403-14 PMID: 4434687
  29. Release of cartilage mucopolysaccharide-degrading neutral protease from human leukocytes.
    J Exp Med. 1973 Aug 1;138(2):461-72 PMID: 4124211
  30. Immunisation with antigen coupled to an immunosorbent.
    Nature. 1974 Feb 15;247(5441):477-8 PMID: 4131936
  31. Neutral proteinases of human spleen. Purification and criteria for homogeneity of elastase and cathepsin G.
    Biochem J. 1976 May 1;155(2):255-63 PMID: 7244
  32. Elastase secretion by stimulated macrophages. Characterization and regulation.
    J Exp Med. 1975 Aug 1;142(2):361-77 PMID: 167096
  33. Inhibition of human leukocyte elastase by peptide chloromethyl ketones.
    FEBS Lett. 1975 Feb 15;50(3):359-61 PMID: 1116607
  34. Subcellular localization and heterogeneity of neutral proteases in neutrophilic polymorphonuclear leukocytes.
    J Exp Med. 1975 Apr 1;141(4):709-23 PMID: 236354
  35. Human pancreatic enzymes: purification and characterization of a nonelastolytic enzyme, protease E. resembling elastase.
    Biochemistry. 1975 Feb 25;14(4):722-30 PMID: 234742
  36. Cathepsin D. Characteristics of immunoinhibition and the confirmation of a role in cartilage breakdown.
    Biochem J. 1971 Jun;123(1):1-13 PMID: 4256870
  37. The interaction of alpha 2-macroglobulin with proteinases. Characteristics and specificity of the reaction, and a hypothesis concerning its molecular mechanism.
    Biochem J. 1973 Aug;133(4):709-24 PMID: 4201304
  38. Human cathepsin G. Catalytic and immunological properties.
    Biochem J. 1976 May 1;155(2):273-8 PMID: 7245
  39. Soluble proteins from human leukocyte granules. I. Esterase activity of cationic proteins.
    Blut. 1973 Jul;27(1):26-32 PMID: 4515732
  40. Purification and properties of a mast cell protease.
    J Biol Chem. 1966 Nov 10;241(21):5090-4 PMID: 5925871
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1550265
Published
1976-05-01
Pages
265-71
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1172831
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1550265 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2