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PMID: 9380714 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Noninfectious virus-like particles produced by Moloney murine leukemia virus-based retrovirus packaging cells deficient in viral envelope become infectious in the presence of lipofection reagents.

Sharma S, Murai F, Miyanohara A, Friedmann T

Abstract

Retrovirus packaging cell lines expressing the Moloney murine leukemia virus gag and pol genes but lacking virus envelope genes produce virus-like particles constitutively, whether or not they express a transcript from an integrated retroviral provirus. In the absence of a proviral transcript, the assembled particles contain processed gag and reverse transcriptase, and particles made by cells expressing an integrated lacZ provirus also contain viral RNA. The virus-like particles from both cell types are enveloped and are secreted/budded into the extracellular space but are noninfectious. Their physicochemical properties are similar to those of mature retroviral particles. The noninfectious gag pol RNA particles can readily be made infectious by the addition of lipofection reagents to produce preparations with titers of up to 10(5) colony-forming units per ml.

MeSH Terms
Cell Line Culture Media, Conditioned Genes, gag Genes, pol Indicators and Reagents Microscopy, Electron Moloney murine leukemia virus/pathogenicity,physiology,ultrastructure RNA, Viral Transfection/methods Virion/pathogenicity,physiology,ultrastructure Virus Assembly
Chemicals
Culture Media, Conditioned Indicators and Reagents RNA, Viral
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sharma S
Department of Pediatrics, Center for Molecular Genetics, University of California at San Diego, La Jolla, California 92093-0634, USA.
Murai F
Miyanohara A
Friedmann T
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
Published
1997-09-30
Pages
10803-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC23491
Subset
IM
Grants
NIDDK NIH HHS · DK-49023-01 · United States
NHLBI NIH HHS · HL-53680-01 · United States
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