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PMID: 9362531 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Regulation of ZAP-70 intracellular localization: visualization with the green fluorescent protein.

The Journal of experimental medicine ·Vol. 186 ·No. 10 ·1997-11-17 ·Pages 1713-24

Sloan-Lancaster J, Zhang W, Presley J, Williams BL, Abraham RT, Lippincott-Schwartz J, Samelson LE

Abstract

To investigate the cellular dynamics of ZAP-70, we have studied the distribution and regulation of its intracellular location using a ZAP-70 green fluorescent protein chimera. Initial experiments in epithelial cells indicated that ZAP-70 is diffusely located throughout the quiescent cell, and accumulates at the plasma membrane upon cellular activation, a phenotype enhanced by the coexpression of Lck and the initiation of ZAP-70 kinase activity. Subsequent studies in T cells confirmed this phenotype. Intriguingly, a large amount of ZAP-70, both chimeric and endogenous, resides in the nucleus of quiescent and activated cells. Nuclear ZAP-70 becomes tyrosine phosphorylated upon stimulation via the T cell receptor, indicating that it may have an important biologic function.

MeSH Terms
Animals Antibodies, Monoclonal/pharmacology Antigens/genetics COS Cells Cell Membrane/enzymology,metabolism Cell Nucleus/chemistry,enzymology,genetics Clone Cells Enzyme Activation/genetics Fluorescent Antibody Technique, Direct Green Fluorescent Proteins Humans Intracellular Fluid/enzymology Jurkat Cells Luminescent Proteins/chemistry,genetics,metabolism Lymphocyte Specific Protein Tyrosine Kinase p56(lck)/genetics,physiology Nuclear Envelope/chemistry Phosphorylation Protein-Tyrosine Kinases/chemistry,genetics,metabolism Receptors, Antigen, T-Cell/immunology,metabolism Recombinant Fusion Proteins/biosynthesis,immunology,metabolism Staining and Labeling Tyrosine/metabolism ZAP-70 Protein-Tyrosine Kinase
Chemicals
Antibodies, Monoclonal Antigens Luminescent Proteins Receptors, Antigen, T-Cell Recombinant Fusion Proteins Green Fluorescent Proteins Tyrosine Protein-Tyrosine Kinases Lymphocyte Specific Protein Tyrosine Kinase p56(lck) ZAP-70 Protein-Tyrosine Kinase ZAP70 protein, human
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Sloan-Lancaster J
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bethesda, Maryland 20892, USA.
Zhang W
Presley J
Williams B L
Abraham R T
Lippincott-Schwartz J
Samelson L E
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.186.10.1713
Published
1997-11-17
Pages
1713-24
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2199132
Subset
IM
Grants
NIGMS NIH HHS · GM47286 · United States
External Links
PubMed source DOI: 10.1084/jem.186.10.1713 PMC Full Text View journal details
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