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PMID: 9359832 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Stress-induced transcription of the clusterin/apoJ gene.

The Biochemical journal ·Vol. 328 ( Pt 1) ·1997-11-15 ·Pages 45-50

Michel D, Chatelain G, North S, Brun G

Abstract

Clusterin/apoJ is an intriguing gene frequently isolated by differential screening in laboratories from different areas of molecular biology, since it is overexpressed in numerous cases of degenerative diseases such as Alzheimer's disease and scrapie. While the dramatic increase of clusterin expression in injured tissues is well established, the molecular basis of the gene induction remains unclear. In this study, we have focused our attention on the only DNA region strictly conserved between clusterin gene proximal promoters from different vertebrate classes. We show that this 14-bp DNA element is specifically recognized by the HSF1 transcription factor and can mediate heat-shock-induced transcription in transient expression assays. Conversely, the avian clusterin proximal promoter, point-mutated at the level of this element, no longer transmits heat-shock activation. These findings provide a possible explanation for the high sensitivity of clusterin expression to environmental changes and allow the classification of clusterin as an extracellular version of heat-shock protein.

MeSH Terms
Animals Base Sequence Carcinoma, Squamous Cell Clusterin DNA-Binding Proteins/genetics,metabolism Glycoproteins/biosynthesis,genetics,metabolism Heat Shock Transcription Factors Hot Temperature Humans Mice Molecular Chaperones Molecular Sequence Data Mutagenesis, Site-Directed Oxidative Stress/genetics Promoter Regions, Genetic Protein Binding/genetics Quail Rats Transcription Factors Transcription, Genetic Tumor Cells, Cultured
Chemicals
CLU protein, human Clu protein, mouse Clusterin DNA-Binding Proteins Glycoproteins HSF1 protein, human Heat Shock Transcription Factors Hsf1 protein, rat Molecular Chaperones Transcription Factors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Michel D
Laboratoire de Biologie Moléculaire, UMR49 CNRS-Ecole Normale Superieure de Lyon, France.
Chatelain G
North S
Brun G
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj3280045
Published
1997-11-15
Pages
45-50
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1218885
Subset
IM
External Links
PubMed source DOI: 10.1042/bj3280045 PMC Full Text View journal details
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