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PMID: 9342376 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Cloning and characterization of the Flavobacterium johnsoniae (Cytophaga johnsonae) gliding motility gene, gldA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 94 ·No. 22 ·1997-10-28 ·Pages 12139-44

Agarwal S, Hunnicutt DW, McBride MJ

Abstract

The mechanism of bacterial gliding motility (active movement over surfaces without the aid of flagella) is not known. A large number of nonmotile mutants of the gliding bacterium Flavobacterium johnsoniae (Cytophaga johnsonae) have been previously isolated, and genetic techniques to analyze these mutants have recently been developed. We complemented a nonmotile mutant of F. johnsoniae (UW102-09) with a library of wild-type DNA by using the shuttle cosmid pCP17. The complementing plasmid (pCP100) contained an insert of 13 kbp, and restored motility to 4 of 61 independently isolated nonmotile mutants. A 1.3-kbp fragment that encompassed a single ORF, gldA, complemented all four mutants. Disruption of the chromosomal copy of gldA in wild-type F. johnsoniae UW101 eliminated gliding motility. The predicted protein produced by gldA has strong sequence similarity to ATP binding cassette transport proteins.

MeSH Terms
ATP-Binding Cassette Transporters/genetics Amino Acid Sequence Bacteria/genetics,virology Bacterial Proteins/genetics Flavobacterium/genetics,virology Gene Library Genes, Bacterial Genetic Complementation Test Molecular Sequence Data Movement/physiology Sequence Analysis, DNA Sequence Homology, Amino Acid
Chemicals
ATP-Binding Cassette Transporters Bacterial Proteins GldA protein, bacteria
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Agarwal S
Department of Biological Sciences, University of Wisconsin, P.O. Box 413, Milwaukee, WI 53201, USA.
Hunnicutt D W
McBride M J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.94.22.12139
Published
1997-10-28
Pages
12139-44
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC23729
Subset
IM
Databases
GENBANK
AF007381
External Links
PubMed source DOI: 10.1073/pnas.94.22.12139 PMC Full Text View journal details
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