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PMID: 9315905 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transforming growth factor-alpha null and senescent mice show decreased neural progenitor cell proliferation in the forebrain subependyma.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 17 ·No. 20 ·1997-10-15 ·Pages 7850-9

Tropepe V, Craig CG, Morshead CM, van der Kooy D

Abstract

The adult mammalian forebrain subependyma contains neural stem cells and their progeny, the constitutively proliferating progenitor cells. Using bromodeoxyuridine labeling to detect mitotically active cells, we demonstrate that the endogenous expression of transforming growth factor-alpha (TGFalpha) is necessary for the full proliferation of progenitor cells localized to the dorsolateral corner of the subependyma and the full production of the neuronal progenitors that migrate to the olfactory bulbs. Proliferation of these progenitor cells also is diminished with age (in 23- to 25-months-old compared with 2- to 4-months-old mice), likely because of a lengthening of the cell cycle. Senescence or the absence of endogenous TGFalpha does not affect the numbers of neural stem cells isolated in vitro in the presence of epidermal growth factor. These results suggest that endogenous TGFalpha and the effects of senescence may regulate the proliferation of progenitor cells in the adult subependyma, but that the number of neural stem cells is maintained throughout life.

MeSH Terms
Aging/physiology Animals Cell Count Cell Cycle Cell Division Cell Movement Culture Techniques Ependyma/cytology Male Mice Mice, Knockout/genetics Neurons/cytology,physiology Olfactory Bulb/cytology Prosencephalon/cytology Stem Cells/cytology Time Factors Transforming Growth Factor alpha/deficiency,genetics
Chemicals
Transforming Growth Factor alpha
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tropepe V
Neurobiology Research Group, Department of Anatomy and Cell Biology, University of Toronto, Toronto, Ontario M5S 1A8, Canada.
Craig C G
Morshead C M
van der Kooy D
References (41)
41 references, click to expand
  1. Methods for determining numbers of cells and synapses: a case for more uniform standards of review.
    J Comp Neurol. 1996 Jan 01;364(1):6-15 PMID: 8789272
  2. The subependymal layer and neighboring region in the brain of the young rat.
    J Comp Neurol. 1972 Nov;146(3):277-302 PMID: 5086674
  3. Is there a neural stem cell in the mammalian forebrain?
    Trends Neurosci. 1996 Sep;19(9):387-93 PMID: 8873356
  4. Estimation of nuclear population from microtome sections.
    Anat Rec. 1946 Feb;94:239-47 PMID: 21015608
  5. Formation and turnover of neurons in young and senescent animals: an electronmicroscopic and morphometric analysis.
    Ann N Y Acad Sci. 1985;457:173-92 PMID: 3913362
  6. A self-renewing multipotential stem cell in embryonic rat cerebral cortex.
    Nature. 1994 Nov 17;372(6503):263-6 PMID: 7969470
  7. Multipotential stem cells from the adult mouse brain proliferate and self-renew in response to basic fibroblast growth factor.
    J Neurosci. 1996 Feb 1;16(3):1091-100 PMID: 8558238
  8. Epidermal growth factor immunoreactive material in the central nervous system: location and development.
    Science. 1984 Jun 8;224(4653):1107-9 PMID: 6144184
  9. Long-distance neuronal migration in the adult mammalian brain.
    Science. 1994 May 20;264(5162):1145-8 PMID: 8178174
  10. A multipotent EGF-responsive striatal embryonic progenitor cell produces neurons and astrocytes.
    J Neurosci. 1992 Nov;12(11):4565-74 PMID: 1432110
  11. Mice with a null mutation of the TGF alpha gene have abnormal skin architecture, wavy hair, and curly whiskers and often develop corneal inflammation.
    Cell. 1993 Apr 23;73(2):249-61 PMID: 8477444
  12. Cellular composition and three-dimensional organization of the subventricular germinal zone in the adult mammalian brain.
    J Neurosci. 1997 Jul 1;17(13):5046-61 PMID: 9185542
  13. Network of tangential pathways for neuronal migration in adult mammalian brain.
    Proc Natl Acad Sci U S A. 1996 Dec 10;93(25):14895-900 PMID: 8962152
  14. Stem cells: attributes, cycles, spirals, pitfalls and uncertainties. Lessons for and from the crypt.
    Development. 1990 Dec;110(4):1001-20 PMID: 2100251
  15. Rat transforming growth factor type 1: structure and relation to epidermal growth factor.
    Science. 1984 Mar 9;223(4640):1079-82 PMID: 6320373
  16. Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system.
    Science. 1992 Mar 27;255(5052):1707-10 PMID: 1553558
  17. Postmitotic death is the fate of constitutively proliferating cells in the subependymal layer of the adult mouse brain.
    J Neurosci. 1992 Jan;12(1):249-56 PMID: 1729437
  18. Proliferative zones of postnatal rat brain express epidermal growth factor receptor mRNA.
    Brain Res. 1995 Jan 23;670(1):157-64 PMID: 7719717
  19. Proliferating subventricular zone cells in the adult mammalian forebrain can differentiate into neurons and glia.
    Proc Natl Acad Sci U S A. 1993 Mar 1;90(5):2074-7 PMID: 8446631
  20. Expression of EGF receptor mRNA in rat nigrostriatal system.
    Neuroreport. 1994 Dec 30;6(1):105-8 PMID: 7703395
  21. In vivo growth factor expansion of endogenous subependymal neural precursor cell populations in the adult mouse brain.
    J Neurosci. 1996 Apr 15;16(8):2649-58 PMID: 8786441
  22. Interkinetic and migratory behavior of a cohort of neocortical neurons arising in the early embryonic murine cerebral wall.
    J Neurosci. 1996 Sep 15;16(18):5762-76 PMID: 8795631
  23. TGF alpha deficiency results in hair follicle and eye abnormalities in targeted and waved-1 mice.
    Cell. 1993 Apr 23;73(2):263-78 PMID: 8477445
  24. Bromodeoxyuridine immunohistochemical determination of the lengths of the cell cycle and the DNA-synthetic phase for an anatomically defined population.
    J Neurocytol. 1989 Jun;18(3):311-8 PMID: 2746304
  25. Clonal and population analyses demonstrate that an EGF-responsive mammalian embryonic CNS precursor is a stem cell.
    Dev Biol. 1996 Apr 10;175(1):1-13 PMID: 8608856
  26. Chain migration of neuronal precursors.
    Science. 1996 Feb 16;271(5251):978-81 PMID: 8584933
  27. Neurogenesis in the dentate gyrus of the adult rat: age-related decrease of neuronal progenitor proliferation.
    J Neurosci. 1996 Mar 15;16(6):2027-33 PMID: 8604047
  28. Differentiation of adult hippocampus-derived progenitors into olfactory neurons in vivo.
    Nature. 1996 Oct 17;383(6601):624-7 PMID: 8857538
  29. A quantitative study of the mitotic activity in the subependymal plate of adult rats.
    Cell Tissue Kinet. 1971 May;4(3):233-8 PMID: 5126175
  30. Restricted proliferation and migration of postnatally generated neurons derived from the forebrain subventricular zone.
    Neuron. 1993 Jul;11(1):173-89 PMID: 8338665
  31. bFGF regulates the proliferative fate of unipotent (neuronal) and bipotent (neuronal/astroglial) EGF-generated CNS progenitor cells.
    Neuron. 1993 Nov;11(5):951-66 PMID: 8240816
  32. The leaving or Q fraction of the murine cerebral proliferative epithelium: a general model of neocortical neuronogenesis.
    J Neurosci. 1996 Oct 1;16(19):6183-96 PMID: 8815900
  33. Genetic deletion of a neural cell adhesion molecule variant (N-CAM-180) produces distinct defects in the central nervous system.
    Neuron. 1993 Dec;11(6):1163-74 PMID: 8274281
  34. Cellular localization of transforming growth factor-alpha mRNA in rat forebrain.
    J Neurochem. 1993 May;60(5):1777-82 PMID: 8473896
  35. The role of polysialic acid in migration of olfactory bulb interneuron precursors in the subventricular zone.
    Neuron. 1996 Apr;16(4):735-43 PMID: 8607992
  36. Autoradiographic and histological studies of postnatal neurogenesis. IV. Cell proliferation and migration in the anterior forebrain, with special reference to persisting neurogenesis in the olfactory bulb.
    J Comp Neurol. 1969 Dec;137(4):433-57 PMID: 5361244
  37. Neural stem cells in the adult mammalian forebrain: a relatively quiescent subpopulation of subependymal cells.
    Neuron. 1994 Nov;13(5):1071-82 PMID: 7946346
  38. Inactivation of the N-CAM gene in mice results in size reduction of the olfactory bulb and deficits in spatial learning.
    Nature. 1994 Feb 3;367(6462):455-9 PMID: 8107803
  39. Localization of cells synthesizing transforming growth factor-alpha mRNA in the mouse brain.
    J Neurosci. 1988 Jun;8(6):1901-4 PMID: 2455024
  40. Epidermal growth factor-like transforming growth factor. II. Interaction with epidermal growth factor receptors in human placenta membranes and A431 cells.
    J Biol Chem. 1983 Nov 25;258(22):13614-20 PMID: 6315706
  41. Embryonic (PSA) N-CAM reveals chains of migrating neuroblasts between the lateral ventricle and the olfactory bulb of adult mice.
    J Comp Neurol. 1995 Jan 2;351(1):51-61 PMID: 7896939
Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
0270-6474
Published
1997-10-15
Pages
7850-9
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6793925
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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