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PMID: 8795631 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Interkinetic and migratory behavior of a cohort of neocortical neurons arising in the early embryonic murine cerebral wall.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 16 ·No. 18 ·1996-09-15 ·Pages 5762-76

Takahashi T, Nowakowski RS, Caviness VS

Abstract

Neocortical neuronogenesis occurs in the pseudostratified ventricular epithelium (PVE) where nuclei of proliferative cells undergo interkinetic nuclear movement. A fraction of daughter cells exits the cell cycle as neurons (the quiescent, or Q, fraction), whereas a complementary fraction remains in the cell cycle (the proliferative, or P, fraction). By means of sequential thymidine and bromodeoxyuridine injections in mouse on embryonic day 14, we have monitored the proliferative and post-mitotic migratory behaviors of 1 and 2 hr cohorts of PVE cells defined by the injection protocols. Soon after mitosis, the Q fraction partitions into a rapidly exiting (up to 50 microns/hr) subpopulation (Qr) and a more slowly exiting (6 microns/hr) subpopulation (Qs). Qr and Qs are separated as two distributions on exit from the ventricular zone with an interpeak distance of approximately 40 microns. Cells in Qr and Qs migrate through the intermediate zone with no significant change in the interpeak distance, suggesting that they migrate at approximately the same velocities. The rate of migration increases with ascent through the intermediate zone (average 2-6.4 microns/hr) slowing only transiently on entry into the developing cortex. Within the cortex, Qr and Qs merge to form a single distribution most concentrated over layer V.

Keywords
NASA Discipline Neuroscience Non-NASA Center
MeSH Terms
Animals Animals, Newborn/growth & development Brain/cytology,embryology,growth & development Cell Movement Cerebral Cortex/cytology Embryo, Mammalian/cytology Embryonic and Fetal Development Mice Mice, Inbred Strains Mitosis Neurons/physiology Time Factors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Takahashi T
Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston 02114, USA.
Nowakowski R S
Caviness V S
Investigators
1 investigators, click to expand
Nowakowski R S
U Med & Dent NJ, Piscataway
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
0270-6474
Published
1996-09-15
Pages
5762-76
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6578981
Subset
IM
Grants
NINDS NIH HHS · R01 NS012005 · United States
NINDS NIH HHS · NS12005 · United States
NINDS NIH HHS · NS28061 · United States
External Links
PubMed source PMC Full Text View journal details
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