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PMID: 9315658 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

In vivo regulation of alternative pre-mRNA splicing by the Clk1 protein kinase.

Molecular and cellular biology ·Vol. 17 ·No. 10 ·1997-10-00 ·Pages 5996-6001

Duncan PI, Stojdl DF, Marius RM, Bell JC

Abstract

Controlled expression of cellular and viral genes through alternative precursor messenger RNA (pre-mRNA) splicing requires serine/arginine-rich (SR) proteins. The Clk1 kinase, which phosphorylates SR proteins, is regulated through alternative splicing of the Clk1 pre-mRNA, yielding mRNAs encoding catalytically active and truncated inactive polypeptides (Clk1 and Clk1T, respectively). We present evidence that Clk1 and Clk1T proteins regulate the splicing of Clk1 and adenovirus pre-mRNAs in vivo. The peptide domain encoded by the alternatively spliced exon of Clk1 is essential for the regulatory activity of the Clk1 kinase. This is the first direct demonstration of an in vivo link between alternative splicing and protein kinase activity.

MeSH Terms
Adenovirus E1A Proteins/genetics Alternative Splicing/physiology Animals Arginine COS Cells Cytomegalovirus/enzymology Exons/genetics Protein Serine-Threonine Kinases/genetics,metabolism Protein-Tyrosine Kinases/genetics,metabolism Proteins/chemistry RNA Precursors/genetics RNA, Viral/genetics Serine
Chemicals
Adenovirus E1A Proteins Proteins RNA Precursors RNA, Viral Serine Arginine Clk dual-specificity kinases Protein-Tyrosine Kinases Protein Serine-Threonine Kinases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Duncan P I
Ottawa Regional Cancer Centre, Ontario, Canada.
Stojdl D F
Marius R M
Bell J C
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.17.10.5996
Published
1997-10-00
Pages
5996-6001
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC232448
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.17.10.5996 PMC Full Text View journal details
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