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PMID: 9311989 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

DBF2, a cell cycle-regulated protein kinase, is physically and functionally associated with the CCR4 transcriptional regulatory complex.

The EMBO journal ·Vol. 16 ·No. 17 ·1997-09-01 ·Pages 5289-98

Liu HY, Toyn JH, Chiang YC, Draper MP, Johnston LH, Denis CL

Abstract

CCR4, a general transcriptional regulator affecting the expression of a number of genes in yeast, forms a multi-subunit complex in vivo. Using the yeast two-hybrid screen, we have identified DBF2, a cell cycle-regulated protein kinase, as a CCR4-associated protein. DBF2 is required for cell cycle progression at the telophase to G1 cell cycle transition. DBF2 co-immunoprecipitated with CCR4 and CAF1/POP2, a CCR4-associated factor, and co-purified with the CCR4 complex. Moreover, a dbf2 disruption resulted in phenotypes and transcriptional defects similar to those observed in strains deficient for CCR4 or CAF1. ccr4 and caf1 mutations, on the other hand, were found to affect cell cycle progression in a manner similar to that observed for dbf2 defects. These data indicate that DBF2 is involved in the control of gene expression and suggest that the CCR4 complex regulates transcription during the late mitotic part of the cell cycle.

MeSH Terms

Cell Cycle/genetics Cell Cycle Proteins Fungal Proteins/isolation & purification,metabolism Gene Expression Regulation, Fungal Mutagenesis Protein Kinases/genetics,isolation & purification,metabolism Protein Serine-Threonine Kinases Ribonucleases Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Transcription Factors/genetics,isolation & purification,metabolism Transcription, Genetic

Chemicals

Cell Cycle Proteins Fungal Proteins Saccharomyces cerevisiae Proteins Transcription Factors Protein Kinases DBF2 protein, S cerevisiae Protein Serine-Threonine Kinases CCR4 protein, S cerevisiae Ribonucleases POP2 protein, S cerevisiae

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Liu H Y

Department of Biochemistry and Molecular Biology, University of New Hampshire, Durham, NH 03824, USA.

Toyn J H

Chiang Y C

Draper M P

Johnston L H

Denis C L

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Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

DOI

10.1093/emboj/16.17.5289

Published

1997-09-01

Pages

5289-98

Language

English

Region

England

NLM ID

8208664

PMCID

PMC1170161

Subset

Grants

NIGMS NIH HHS · GM 41215 · United States

External Links

PubMed source DOI: 10.1093/emboj/16.17.5289 PMC Full Text View journal details

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DBF2, a cell cycle-regulated protein kinase, is physically and functionally associated with the CCR4 transcriptional regulatory complex.

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