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PMID: 8035801 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

SPT10 and SPT21 are required for transcription of particular histone genes in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 14 ·No. 8 ·1994-08-00 ·Pages 5223-8

Dollard C, Ricupero-Hovasse SL, Natsoulis G, Boeke JD, Winston F

Abstract

The Saccharomyces cerevisiae genome contains four loci that encode histone proteins. Two of these loci, HTA1-HTB1 and HTA2-HTB2, each encode histones H2A and H2B. The other two loci, HHT1-HHF1 and HHT2-HHF2, each encode histones H3 and H4. Because of their redundancy, deletion of any one histone locus does not cause lethality. Previous experiments demonstrated that mutations at one histone locus, HTA1-HTB1, do cause lethality when in conjunction with mutations in the SPT10 gene. SPT10 has been shown to be required for normal levels of transcription of several genes in S. cerevisiae. Motivated by this double-mutant lethality, we have now investigated the interactions of mutations in SPT10 and in a functionally related gene, SPT21, with mutations at each of the four histone loci. These experiments have demonstrated that both SPT10 and SPT21 are required for transcription at two particular histone loci, HTA2-HTB2 and HHF2-HHT2, but not at the other two histone loci. These results suggest that under some conditions, S. cerevisiae may control the level of histone proteins by differential expression of its histone genes.

Related Genes
HHF1 HHF2 SPT10 SPT21
MeSH Terms
Fungal Proteins/genetics,physiology Gene Expression Regulation, Fungal Histone Acetyltransferases Histones/genetics RNA, Fungal/genetics RNA, Messenger/genetics Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Transcription Factors Transcription, Genetic
Chemicals
Fungal Proteins Histones RNA, Fungal RNA, Messenger SPT21 protein, S cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Histone Acetyltransferases SPT10 protein, S cerevisiae
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Dollard C
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
Ricupero-Hovasse S L
Natsoulis G
Boeke J D
Winston F
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.14.8.5223-5228.1994
Published
1994-08-00
Pages
5223-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359041
Subset
IM
Grants
NCI NIH HHS · CA16519 · United States
NIGMS NIH HHS · GM32967 · United States
NIGMS NIH HHS · GM36481 · United States
External Links
PubMed source DOI: 10.1128/mcb.14.8.5223-5228.1994 PMC Full Text View journal details
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