Home LiteratureArticle Details
PMID: 9311899 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Partial resistance to gD-mediated interference conferred by mutations affecting herpes simplex virus type 1 gC and gK.

Journal of virology ·Vol. 71 ·No. 10 ·1997-10-00 ·Pages 8024-8

Pertel PE, Spear PG

Abstract

Cells expressing herpes simplex virus (HSV) gD can be resistant to HSV entry as a result of gD-mediated interference. HSV strains differ in sensitivity to this interference, which blocks viral penetration but not binding. Previous studies have shown that mutations or variations in virion-associated gD can confer resistance to gD-mediated interference. Here we show that HSV-1 mutants selected for enhanced ability to bind and penetrate in the presence of inhibitory concentrations of heparin were partially resistant to gD-mediated interference. The resistance was largely due to the presence of two mutations: one in gC (the major heparin-binding glycoprotein) resulting in the absence of gC expression and the other in gK resulting in a syncytial phenotype. The results imply that heparin selected for mutants with altered postbinding requirements for entry. Resistance to gD-mediated interference conferred by mutations affecting gC and gK has not been previously described.

MeSH Terms
Animals CHO Cells Cricetinae Genetic Variation Heparin/pharmacology Herpesvirus 1, Human/drug effects,genetics,physiology Humans Mutagenesis Mutagenesis, Site-Directed Polymerase Chain Reaction Tumor Cells, Cultured Viral Envelope Proteins Viral Proteins/biosynthesis,metabolism Virion/physiology
Chemicals
UL53 protein, Human herpesvirus 1 Viral Envelope Proteins Viral Proteins glycoprotein gG-1, herpes simplex virus type 1 Heparin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pertel P E
Department of Medicine, Northwestern University Medical School, Chicago, Illinois 60611, USA.
Spear P G
References (47)
47 references, click to expand
  1. Anti-glycoprotein D antibodies that permit adsorption but block infection by herpes simplex virus 1 prevent virion-cell fusion at the cell surface.
    Proc Natl Acad Sci U S A. 1987 Aug;84(15):5454-8 PMID: 3037552
  2. INHIBITION OF HERPES VIRUS BY NATURAL AND SYNTHETIC ACID POLYSACCHARIDES.
    Proc Soc Exp Biol Med. 1964 May;116:140-4 PMID: 14200087
  3. A herpes simplex virus mutant in which glycoprotein D sequences are replaced by beta-galactosidase sequences binds to but is unable to penetrate into cells.
    J Virol. 1988 May;62(5):1486-94 PMID: 2833603
  4. Reduced yield of infectious pseudorabies virus and herpes simplex virus from cell lines producing viral glycoprotein gp50.
    J Virol. 1988 Jun;62(6):2196-9 PMID: 2835521
  5. Role of glycoprotein B of herpes simplex virus type 1 in viral entry and cell fusion.
    J Virol. 1988 Aug;62(8):2596-604 PMID: 2839688
  6. Initial interaction of herpes simplex virus with cells is binding to heparan sulfate.
    J Virol. 1989 Jan;63(1):52-8 PMID: 2535752
  7. Herpes simplex virus glycoprotein D mediates interference with herpes simplex virus infection.
    J Virol. 1989 Feb;63(2):819-27 PMID: 2536105
  8. Fine mapping of antigenic site II of herpes simplex virus glycoprotein D.
    J Virol. 1989 May;63(5):2325-34 PMID: 2467994
  9. Bovine cells expressing bovine herpesvirus 1 (BHV-1) glycoprotein IV resist infection by BHV-1, herpes simplex virus, and pseudorabies virus.
    J Virol. 1990 Oct;64(10):4866-72 PMID: 2168973
  10. Molecular cloning, sequencing, and expression of functional bovine herpesvirus 1 glycoprotein gIV in transfected bovine cells.
    J Virol. 1990 Oct;64(10):5132-42 PMID: 2168991
  11. Glycoprotein D of herpes simplex virus encodes a domain which precludes penetration of cells expressing the glycoprotein by superinfecting herpes simplex virus.
    J Virol. 1990 Dec;64(12):6070-9 PMID: 2173780
  12. Glycoprotein C of herpes simplex virus type 1 plays a principal role in the adsorption of virus to cells and in infectivity.
    J Virol. 1991 Mar;65(3):1090-8 PMID: 1847438
  13. Modified entry and syncytium formation by herpes simplex virus type 1 mutants selected for resistance to heparin inhibition.
    Virology. 1996 Dec 1;226(1):22-33 PMID: 8941319
  14. Glycoprotein D of herpes simplex virus (HSV) binds directly to HVEM, a member of the tumor necrosis factor receptor superfamily and a mediator of HSV entry.
    J Virol. 1997 Aug;71(8):6083-93 PMID: 9223502
  15. [CYTOPATHIC EFFECT AND PLAQUE MORPHOLOGY OF VARIOUS HERPES SIMPLEX VIRUS STRAINS].
    Arch Gesamte Virusforsch. 1963 Aug 26;13:529-40 PMID: 14078843
  16. THE EFFECT OF HEPARIN ON HERPES SIMPLEX VIRUS.
    Virology. 1963 Dec;21:661-2 PMID: 14100619
  17. Glycosaminoglycans: molecular properties, protein interactions, and role in physiological processes.
    Physiol Rev. 1991 Apr;71(2):481-539 PMID: 2006221
  18. Penetration of cells by herpes simplex virus does not require a low pH-dependent endocytic pathway.
    Virus Res. 1991 Mar;18(2-3):271-90 PMID: 1645908
  19. Construction and properties of a mutant of herpes simplex virus type 1 with glycoprotein H coding sequences deleted.
    J Virol. 1992 Jan;66(1):341-8 PMID: 1309250
  20. Cell surface receptors for herpes simplex virus are heparan sulfate proteoglycans.
    J Cell Biol. 1992 Mar;116(5):1273-81 PMID: 1310996
  21. Herpes simplex virus type 1 entry through a cascade of virus-cell interactions requires different roles of gD and gH in penetration.
    J Virol. 1992 Aug;66(8):5002-12 PMID: 1321283
  22. Herpes simplex virus infection and propagation in a mouse L cell mutant lacking heparan sulfate proteoglycans.
    J Virol. 1993 Jan;67(1):93-100 PMID: 8380101
  23. A mutant herpes simplex virus type 1 unable to express glycoprotein L cannot enter cells, and its particles lack glycoprotein H.
    J Virol. 1993 Apr;67(4):2285-97 PMID: 8383241
  24. Resistance and susceptibility of bovine cells expressing herpesviral glycoprotein D homologs to herpesviral infections.
    Virology. 1993 May;194(1):365-9 PMID: 8386880
  25. Glycoproteins gIII and gp50 play dominant roles in the biphasic attachment of pseudorabies virus.
    Virology. 1993 Jun;194(2):654-64 PMID: 8389078
  26. Herpes simplex virus type 1 and pseudorabies virus bind to a common saturable receptor on Vero cells that is not heparan sulfate.
    J Virol. 1993 Sep;67(9):5088-97 PMID: 8394437
  27. Herpesvirus-induced cell fusion that is dependent on cell surface heparan sulfate or soluble heparin.
    J Virol. 1994 Feb;68(2):1224-8 PMID: 8289356
  28. Single amino acid substitutions in gD of herpes simplex virus 1 confer resistance to gD-mediated interference and cause cell-type-dependent alterations in infectivity.
    Virology. 1994 Feb 15;199(1):67-80 PMID: 8116256
  29. Glycoprotein C-independent binding of herpes simplex virus to cells requires cell surface heparan sulphate and glycoprotein B.
    J Gen Virol. 1994 Jun;75 ( Pt 6):1211-22 PMID: 8207388
  30. Herpes simplex virus glycoprotein D acquires mannose 6-phosphate residues and binds to mannose 6-phosphate receptors.
    J Biol Chem. 1994 Jun 24;269(25):17067-74 PMID: 8006011
  31. Role of mannose-6-phosphate receptors in herpes simplex virus entry into cells and cell-to-cell transmission.
    J Virol. 1995 Jun;69(6):3517-28 PMID: 7745699
  32. Viral determinants of the variable sensitivity of herpes simplex virus strains to gD-mediated interference.
    J Virol. 1995 Aug;69(8):5171-6 PMID: 7609090
  33. Anti-idiotypic antibodies mimicking glycoprotein D of herpes simplex virus identify a cellular protein required for virus spread from cell to cell and virus-induced polykaryocytosis.
    Proc Natl Acad Sci U S A. 1996 Mar 5;93(5):1836-40 PMID: 8700845
  34. Herpes simplex virus-1 entry into cells mediated by a novel member of the TNF/NGF receptor family.
    Cell. 1996 Nov 1;87(3):427-36 PMID: 8898196
  35. Electron microscopy of herpes simplex virus. I. Entry.
    J Virol. 1968 May;2(5):507-16 PMID: 4301316
  36. Properties of plaque variants of herpes virus hominis strains of genital origin.
    Arch Gesamte Virusforsch. 1972;37(4):308-15 PMID: 4339105
  37. Comparison of the virion proteins specified by herpes simplex virus types 1 and 2.
    J Virol. 1975 Nov;16(5):1327-31 PMID: 171455
  38. Cell fusion induced by herpes simplex virus is promoted and suppressed by different viral glycoproteins.
    Proc Natl Acad Sci U S A. 1977 Sep;74(9):3913-7 PMID: 198812
  39. Membrane proteins specified by herpes simplex viruses. III. Role of glycoprotein VP7(B2) in virion infectivity.
    J Virol. 1979 Mar;29(3):1149-58 PMID: 221669
  40. Immunoglobulin G(Fc)-binding receptors on virions of herpes simplex virus type 1 and transfer of these receptors to the cell surface by infection.
    J Virol. 1980 May;34(2):512-20 PMID: 6246278
  41. Structural analysis of the capsid polypeptides of herpes simplex virus types 1 and 2.
    J Virol. 1980 May;34(2):521-31 PMID: 6154808
  42. Characterization of the herpes simplex virion-associated factor responsible for the induction of alpha genes.
    J Virol. 1983 May;46(2):371-7 PMID: 6302308
  43. Physical mapping of the mutation in an antigenic variant of herpes simplex virus type 1 by use of an immunoreactive plaque assay.
    J Virol. 1983 May;46(2):649-52 PMID: 6302327
  44. Characterisation of a herpes simplex virus type 1 mutant which has a temperature-sensitive defect in penetration of cells and assembly of capsids.
    Virology. 1984 Oct 30;138(2):246-59 PMID: 6093367
  45. Identification of herpes simplex virus DNA sequences which encode a trans-acting polypeptide responsible for stimulation of immediate early transcription.
    J Mol Biol. 1984 Nov 25;180(1):1-19 PMID: 6096556
  46. An analysis of the biological properties of monoclonal antibodies against glycoprotein D of herpes simplex virus and identification of amino acid substitutions that confer resistance to neutralization.
    J Gen Virol. 1986 Jun;67 ( Pt 6):1001-13 PMID: 2423636
  47. Entry of herpes simplex virus 1 in BJ cells that constitutively express viral glycoprotein D is by endocytosis and results in degradation of the virus.
    J Virol. 1988 Jan;62(1):159-67 PMID: 2824844
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.71.10.8024-8028.1997
Published
1997-10-00
Pages
8024-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC192166
Subset
IM
Grants
NIAID NIH HHS · R01 AI 36293-03 · United States
External Links
PubMed source DOI: 10.1128/JVI.71.10.8024-8028.1997 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2