Reduced yield of infectious pseudorabies virus and herpes simplex virus from cell lines producing viral glycoprotein gp50.

Journal of virology ·Vol. 62 ·No. 6 ·1988-06-00 ·Pages 2196-9

Petrovskis EA, Meyer AL, Post LE

Abstract

Pseudorabies virus (PRV) glycoprotein gp50 is the homolog of herpes simplex virus (HSV) glycoprotein D. Several cell lines that constitutively synthesize gp50 were constructed. Vero cells, HeLa cells, and pig kidney (MVPK) cells that produce gp50 all gave reduced yields of PRV and HSV progeny viruses when compared with the parent cell line or the same cell line transfected to produce a different protein. The reduction in virus yield was greatest at low multiplicities of infection. The Vero and HeLa cells that produce gp50 showed an even greater reduction in HSV yield than in PRV yield. This phenomenon may be an example in a herpesvirus of the interference observed in retroviruses or cross-protection in plant virus systems.

MeSH Terms

Animals Cloning, Molecular HeLa Cells Herpesvirus 1, Suid/genetics,growth & development Simplexvirus/genetics,growth & development Vero Cells Viral Envelope Proteins/physiology Virus Replication

Chemicals

Viral Envelope Proteins

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Petrovskis E A

Upjohn Company, Kalamazoo, Michigan 49007.

Meyer A L

Post L E

References (24)

24 references, click to expand

The mechanism of interference between an avian leukosis virus and Rous sarcoma virus. I. Establishment of interference.
Virology. 1966 Aug;29(4):628-41 PMID: 4287698
Entry of herpes simplex virus 1 in BJ cells that constitutively express viral glycoprotein D is by endocytosis and results in degradation of the virus.
J Virol. 1988 Jan;62(1):159-67 PMID: 2824844
A new technique for the assay of infectivity of human adenovirus 5 DNA.
Virology. 1973 Apr;52(2):456-67 PMID: 4705382
Friend murine leukemia virus-induced leukemia is associated with the formation of mink cell focus-inducing viruses and is blocked in mice expressing endogenous mink cell focus-inducing xenotropic viral envelope genes.
J Exp Med. 1981 Sep 1;154(3):907-20 PMID: 6268733
Host Susceptibility to endogenous viruses: defective, glycoprotein-expressing proviruses interfere with infections.
J Virol. 1981 Dec;40(3):745-51 PMID: 6275116
Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.
J Mol Appl Genet. 1982;1(4):327-41 PMID: 6286831
Normal DBA/2 mouse cells synthesize a glycoprotein which interferes with MCF virus infection.
Virology. 1982 Nov;123(1):139-51 PMID: 6959413
Cellular expression of murine leukemia virus gp70-related antigen on thymocytes of uninfected mice correlates with Fv-4 gene-controlled resistance to Friend leukemia virus infection.
Virology. 1983 Jul 15;128(1):127-39 PMID: 6192586
Anti-gD monoclonal antibodies inhibit cell fusion induced by herpes simplex virus type 1.
Virology. 1983 Aug;129(1):218-24 PMID: 6310880
Isolation, characterization, and physical mapping of a pseudorabies virus mutant containing antigenically altered gp50.
J Virol. 1984 Jul;51(1):57-62 PMID: 6202885
Mapping and sequence of the gene for the pseudorabies virus glycoprotein which accumulates in the medium of infected cells.
J Virol. 1985 Apr;54(1):21-9 PMID: 2983115
Specificities of monoclonal and polyclonal antibodies that inhibit adsorption of herpes simplex virus to cells and lack of inhibition by potent neutralizing antibodies.
J Virol. 1985 Aug;55(2):475-82 PMID: 2991570
Characterization of a molecularly cloned retroviral sequence associated with Fv-4 resistance.
J Virol. 1985 Sep;55(3):768-77 PMID: 2991595
Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene.
Science. 1986 May 9;232(4751):738-43 PMID: 3457472
Characterization of a pseudorabies virus glycoprotein gene with homology to herpes simplex virus type 1 and type 2 glycoprotein C.
J Virol. 1986 May;58(2):339-47 PMID: 3009851
DNA sequence of the gene for pseudorabies virus gp50, a glycoprotein without N-linked glycosylation.
J Virol. 1986 Aug;59(2):216-23 PMID: 3016293
Use of lambda gt11 to isolate genes for two pseudorabies virus glycoproteins with homology to herpes simplex virus and varicella-zoster virus glycoproteins.
J Virol. 1986 Oct;60(1):185-93 PMID: 3018284
Identification of two forms of an endogenous murine retroviral env gene linked to the Rmcf locus.
J Virol. 1987 Jan;61(1):29-34 PMID: 3023705
Evidence that neomycin inhibits HSV 1 infection of BHK cells.
Biochem Biophys Res Commun. 1986 Nov 26;141(1):198-203 PMID: 3026374
Molecular characterization of the Akvr-1 restriction gene: a defective endogenous retrovirus-borne gene identical to Fv-4r.
J Virol. 1987 Feb;61(2):308-14 PMID: 3027362
Anti-glycoprotein D antibodies that permit adsorption but block infection by herpes simplex virus 1 prevent virion-cell fusion at the cell surface.
Proc Natl Acad Sci U S A. 1987 Aug;84(15):5454-8 PMID: 3037552
The pseudorabies virus gII gene is closely related to the gB glycoprotein gene of herpes simplex virus.
J Virol. 1987 Sep;61(9):2691-701 PMID: 3039163
Evaluation of pseudorabies virus glycoprotein gp50 as a vaccine for Aujeszky's disease in mice and swine: expression by vaccinia virus and Chinese hamster ovary cells.
J Virol. 1987 Dec;61(12):3977-82 PMID: 2824827
The mechanism of interference between an avian leukosis virus and Rous sarcoma virus. II. Early steps of infection by RSV of cells under conditions of interference.
Virology. 1966 Aug;29(4):642-53 PMID: 4287699

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.62.6.2196-2199.1988

Published

1988-06-00

Pages

2196-9

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC253329

Subset

External Links

PubMed source DOI: 10.1128/JVI.62.6.2196-2199.1988 PMC Full Text View journal details

Analysis Services

Reduced yield of infectious pseudorabies virus and herpes simplex virus from cell lines producing viral glycoprotein gp50.

Contact

WeChat Official Account