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PMID: 9294147 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Memory B cells are biased towards terminal differentiation: a strategy that may prevent repertoire freezing.

The Journal of experimental medicine ·Vol. 186 ·No. 6 ·1997-09-15 ·Pages 931-40

Arpin C, Banchereau J, Liu YJ

Abstract

Isolation of large numbers of surface IgD+CD38- naive and surface IgD-CD38- memory B cells allowed us to study the intrinsic differences between these two populations. Upon in vitro culture with IL-2 and IL-10, human CD40-activated memory B cells undergo terminal differentiation into plasma cells more readily than do naive B cells, as they give rise to five- to eightfold more plasma cells and three- to fourfold more secreted immunoglobulins. By contrast, naive B cells give rise to a larger number of nondifferentiated B blasts. Saturating concentrations of CD40 ligand, which fully inhibit naive B cell differentiation, only partially affect that of memory B cells. The propensity of memory B cells to undergo terminal plasma cell differentiation may explain the extensive extra follicular plasma cell reaction and the limited germinal center reaction observed in vivo after secondary immunizations, which contrast with primary responses in carrier-primed animals. This unique feature of memory B cells may confer two important capacities to the immune system: (a) the rapid generation of a large number of effector cells to efficiently eliminate the pathogens; and (b) the prevention of the overexpansion and chronic accumulation of one particular memory B cell clone that would freeze the available peripheral repertoire.

MeSH Terms
Animals B-Lymphocytes/cytology,immunology CD40 Antigens/metabolism CD40 Ligand Cell Differentiation Child Humans Immunologic Memory In Vitro Techniques Interleukin-10/pharmacology Interleukin-2/pharmacology Lymphocyte Activation Membrane Glycoproteins/metabolism Mice Palatine Tonsil/cytology,immunology Plasma Cells/cytology,immunology Rats Receptors, Antigen, B-Cell/metabolism
Chemicals
CD40 Antigens Interleukin-2 Membrane Glycoproteins Receptors, Antigen, B-Cell Interleukin-10 CD40 Ligand
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Arpin C
Schering-Plough, Laboratory for Immunological Research, 69571 Dardilly, France.
Banchereau J
Liu Y J
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.186.6.931
Published
1997-09-15
Pages
931-40
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2199043
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.186.6.931 PMC Full Text View journal details
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