Transposon tagging in rice.

Plant molecular biology ·Vol. 35 ·No. 1-2 ·1997-09-00 ·Pages 219-29

Izawa T, Ohnishi T, Nakano T, Ishida N, Enoki H, Hashimoto H, Itoh K, Terada R, Wu C, Miyazaki C, Endo T, Iida S, Shimamoto K

Abstract

To develop an efficient gene isolation method for rice we introduced the maize Ac/Ds system into rice. Extensive analysis of their behavior in rice for several generations indicated that Ac and Ds in the presence of Ac transposase gene actively transpose in rice. A wide spectrum of mutations affecting growth, morphogenesis, flowering time and disease resistance have been obtained in the population carrying Ac/Ds and some of them were genetically analyzed. Main efforts are currently being made to isolate genes responsible these mutations. In addition, a number of Ac/Ds were mapped on chromosomes and mapped elements will be used in the future for directed tagging of genes with known chromosomal positions.

MeSH Terms

Cloning, Molecular DNA Transposable Elements Genes, Plant Genetic Markers Oryza/genetics

Chemicals

DNA Transposable Elements Genetic Markers

Authors & Affiliations

13 authors, click to expand affiliations / ORCID

Izawa T

Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology, Ikoma, Japan.

Ohnishi T

Nakano T

Ishida N

Enoki H

Hashimoto H

Itoh K

Terada R

Wu C

Miyazaki C

Endo T

Iida S

Shimamoto K

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Article Info

Journal

Plant molecular biology

Abbr.

Plant Mol Biol

ISSN

0167-4412

Published

1997-09-00

Pages

219-29

Language

English

Region

Netherlands

NLM ID

9106343

Subset

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Transposon tagging in rice.

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